Literature DB >> 1054847

Translation in vitro of rat brain messenger RNA coding for tubulin and actin.

I Gozes, H Schmitt, U Z Littauer.   

Abstract

A partially purified fraction of poly(a)-rich brain mRNA coding for tubulin and actin was obtained by stepwise elution from an oligo(deoxythymidylate)-cellulose column and was efficiently translated in a wheat-germ cell-free system. The newly synthesized tubulin and actin migrated along with the authentic proteins on sodium dodecyl sulfate polyacrylamide gels and on sodium dodecyl sulfate-urea polyacrylamide gels, where tubulin alpha- and beta-subunits are separated. The two proteins synthesized in vitro were found to be biologically active; they could be induced to polymerize and were both precipitated by vinblastine. In addition, specific binding of tubulin to an affinity column of colchicine-Sepharose and actin to myosin were demonstrated.

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Year:  1975        PMID： 1054847      PMCID： PMC432383          DOI： 10.1073/pnas.72.2.701

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Actomyosin-like protein in brain.

Authors:  S Berl; S Puszkin; W J Nicklas
Journal:  Science       Date:  1973-02-02       Impact factor: 47.728

2.  Mg2+ -Ca2+ -activated adenosine triphosphatase system isolated from mammalian brain.

Authors:  S Berl; S Puszkin
Journal:  Biochemistry       Date:  1970-05-12       Impact factor: 3.162

3.  In vitro synthesis of mouse neuroblastoma tubulin.

Authors:  G Wiche; C Zomzely-Neurath; A J Blume
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205

4.  Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

Authors:  B E Roberts; B M Paterson
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

5.  Microtubule assembly in the absence of added nucleotides.

Authors:  M L Shelanski; F Gaskin; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

6.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

7.  Isolation of tubulin from the nervous system of Aplysia californica.

Authors:  M Jacobs; A V Brown; G L Brown
Journal:  FEBS Lett       Date:  1972-07-15       Impact factor: 4.124

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

10.  Determination of actin messenger RNA in cultures of differentiating embryonic chick skeletal muscle.

Authors:  B M Paterson; B E Roberts; D Yaffe
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205
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2.  Chick cytoplasmic actin and muscle actin have different structural genes.

Authors:  R V Storti; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

3.  Pregrowth hormone: product of the translation in vitro of messenger RNA coding for growth hormone.

Authors:  P M Sussman; R J Tushinski; F C Bancroft
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

4.  Biosynthesis of mouse interferon by translation of its messenger RNA in a cell-free system.

Authors:  M N Thang; D C Thang; E De Maeyer; L Montagnier
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

5.  An assay for albumin messenger RNA in an vitro protein synthesizing system from wheat germ.

Authors:  J A Peterson
Journal:  Nucleic Acids Res       Date:  1976-06       Impact factor: 16.971

6.  Cell-free synthesis of rat preproinsulins: characterization and partial amino acid sequence determination.

Authors:  S J Chan; P Keim; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

7.  Developmental changes in the composition of polyadenylated RNA isolated from free and membrane-bound polyribosomes of the rat forebrain, analysed by translation in vitro.

Authors:  C Hall; L Lim
Journal:  Biochem J       Date:  1981-04-15       Impact factor: 3.857

8.  Identification of an actin-like protein and of its messenger ribonucleic acid in Saccharomyces cerevisiae.

Authors:  R D Water; J R Pringle; L J Kleinsmith
Journal:  J Bacteriol       Date:  1980-12       Impact factor: 3.490

9.  Tau pathology: predictive diagnostics, targeted preventive and personalized medicine and application of advanced research in medical practice.

Authors:  Illana Gozes
Journal:  EPMA J       Date:  2010-06-12       Impact factor: 6.543
  9 in total

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