Literature DB >> 986643

An assay for albumin messenger RNA in an vitro protein synthesizing system from wheat germ.

J A Peterson.   

Abstract

The synthesis of serum albumin in an in vitro protein-synthesizing system from wheat germ stimulated with rat liver polysomal RNA is demonstrated by immunoprecipitation. The newly synthesized albumin has the same electrophoretic mobility as rat serum albumin. There is a linear increase in precursor incorporation into total protein and albumin with increasing RNA concentration. Potassium and magnesium optima for albumin synthesis are different from those for total protein synthesis.

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Year:  1976        PMID： 986643      PMCID： PMC343002          DOI： 10.1093/nar/3.6.1427

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  The ratio of albumin synthesis to total protein synthesis in normal rat liver, in host liver, and in Morris hepatoma 9121.

Authors:  H M Rotermund; G Schreiber; H Maeno; U Weinssen; K Weigand
Journal:  Cancer Res       Date:  1970-08       Impact factor: 12.701

3.  Regulation of hemoglobin synthesis. Effect of concentration of messenger ribonucleic acid, ribosome subunits, initiation factors, and salts on ratio of alpha and beta chains synthesized in vitro.

Authors:  W L McKeehan
Journal:  J Biol Chem       Date:  1974-10-25       Impact factor: 5.157

4.  Isolation and in vitro translation of soybean leghaemoglobin mRNA.

Authors:  D P Verma; D T Nash; H M Schulman
Journal:  Nature       Date:  1974-09-06       Impact factor: 49.962

5.  The proption of the incorporation of leucine into albumin to that into totaprotein in rat liver and hepatoma Morris 5123 TC.

Authors:  G Schreiber; H M Rotermund; H Maeno; K Weigand; R Lesch
Journal:  Eur J Biochem       Date:  1969-09

6.  Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

Authors:  B E Roberts; B M Paterson
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

7.  Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors:  R D Palmiter
Journal:  Biochemistry       Date:  1974-08-13       Impact factor: 3.162

8.  Protein synthesis with messenger ribonucleic acid fractions from membrane-bound and free liver polysomes. Translation characteristics of liver polysomal ribonucleic acids and evidence for albumin production in a messenger-dependent reticulocyte cell-free system.

Authors:  D A Shafritz
Journal:  J Biol Chem       Date:  1974-01-10       Impact factor: 5.157

9.  The wheat embryo cell-free system.

Authors:  A Marcus; D Efron; D P Weeks
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

10.  Discontinuous variability, in the form of a geometric progression, of albumin production in hepatoma and hybrid cells.

Authors:  J A Peterson
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205
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  3 in total

1.  Distribution of rat liver albumin mRNA membrane-bound and free in polyribosomes as determined by molecular hybridization.

Authors:  S H Yap; R K Strair; D A Shafritz
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

2.  Absence of albumin mRNA in the liver of analbuminemic rats.

Authors:  H Esumi; M Okui; S Sato; T Sugimura; S Nagase
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

3.  Regulation of albumin synthesis in rat liver.

Authors:  D A Shafritz; S H Yap; R K Strair
Journal:  Mol Biol Rep       Date:  1979-05-31       Impact factor: 2.316
  3 in total

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