Literature DB >> 6774336

Two control regions for eukaryotic tRNA gene transcription.

D DeFranco, O Schmidt, D Söll.   

Abstract

Two Drosophila tRNALys genes with identical coding sequences were shown to transcribe with very different efficiences in nuclear extracts from Xenopus oocytes. The use of recombinant plasmids in which the 5'-flanking sequences of these genes were either "switched" or replaced by defined pBR322 sequences revealed two control regions for tRNA gene transcription. An internal control region comprising the mature tRNA coding sequence (and possibly its 3'-flanking sequences) is sufficient for transcription initiation, and an external control region comprising the 5'-flanking sequences represses this transcription. All transcripts have short leader sequences. Altered precursor tRNAs transcribed from truncated tRNALys genes (missing a single base pair in the acceptor stem) are not processed well in vitro.

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Year:  1980        PMID： 6774336      PMCID： PMC349616          DOI： 10.1073/pnas.77.6.3365

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.

Authors:  S Silverman; O Schmidt; D Söll; B Hovemann
Journal:  J Biol Chem       Date:  1979-10-25       Impact factor: 5.157

2.  Isolation of yeast tRNALeu genes. DNA sequence of a cloned tRNALeu3 gene.

Authors:  A Venegas; M Quiroga; J Zaldivar; W J Rutter; P Valenzuela
Journal:  J Biol Chem       Date:  1979-12-25       Impact factor: 5.157

3.  Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.

Authors:  D R Engelke; S Y Ng; B S Shastry; R G Roeder
Journal:  Cell       Date:  1980-03       Impact factor: 41.582

4.  A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.

Authors:  A Kressmann; H Hofstetter; E Di Capua; R Grosschedl; M L Birnstiel
Journal:  Nucleic Acids Res       Date:  1979-12-11       Impact factor: 16.971

5.  Analysis of a drosophila tRNA gene cluster.

Authors:  B Hovemann; S Sharp; H Yamada; D Söll
Journal:  Cell       Date:  1980-04       Impact factor: 41.582

6.  A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.

Authors:  S Sakonju; D F Bogenhagen; D D Brown
Journal:  Cell       Date:  1980-01       Impact factor: 41.582

7.  A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.

Authors:  D F Bogenhagen; S Sakonju; D D Brown
Journal:  Cell       Date:  1980-01       Impact factor: 41.582

8.  Transcription initiation of Xenopus 5S ribosomal RNA genes in vitro.

Authors:  L J Korn; E H Birkenmeier; D D Brown
Journal:  Nucleic Acids Res       Date:  1979-10-25       Impact factor: 16.971

9.  Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro.

Authors:  R L Garber; L P Gage
Journal:  Cell       Date:  1979-11       Impact factor: 41.582

10.  The primary transcription product of a silkworm alanine tRNA gene: identification of in vitro sites of initiation, termination and processing.

Authors:  O Hagenbüchle; D Larson; G I Hall; K U Sprague
Journal:  Cell       Date:  1979-12       Impact factor: 41.582
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  61 in total

1.  Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes.

Authors:  G A Kassavetis; D L Riggs; R Negri; L H Nguyen; E P Geiduschek
Journal:  Mol Cell Biol       Date:  1989-06       Impact factor: 4.272

2.  The role of the 5'-flanking sequence of a human tRNA(Glu) gene in modulation of its transcriptional activity in vitro.

Authors:  E S Gonos; J P Goddard
Journal:  Biochem J       Date:  1990-12-15       Impact factor: 3.857

3.  Centromeric polymerase III transcription units in Chironomus pallidivittatus.

Authors:  C Rovira; J E Edström
Journal:  Nucleic Acids Res       Date:  1996-05-01       Impact factor: 16.971

4.  Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII.

Authors:  M D Rosa; E Gottlieb; M R Lerner; J A Steitz
Journal:  Mol Cell Biol       Date:  1981-09       Impact factor: 4.272

5.  A human tRNA(iMet) gene produces multiple transcripts.

Authors:  C L Vnencak-Jones; S Z Wahab; Z E Zehner; W M Holmes
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

6.  In vitro transcription of RU, a middle repetitive element of the rat genome.

Authors:  G Page; M Salvato; H M Goodman
Journal:  Nucleic Acids Res       Date:  1986-01-24       Impact factor: 16.971

7.  Effects of altered 5'-flanking sequences on the in vivo expression of a Saccharomyces cerevisiae tRNATyr gene.

Authors:  K J Shaw; M V Olson
Journal:  Mol Cell Biol       Date:  1984-04       Impact factor: 4.272

8.  A minor class of 5S rRNA genes in Saccharomyces cerevisiae X2180-1B, one member of which lies adjacent to a Ty transposable element.

Authors:  P W Piper; A Lockheart; N Patel
Journal:  Nucleic Acids Res       Date:  1984-05-25       Impact factor: 16.971

9.  Structural organization of transposable element mdg4 from Drosophila melanogaster and a nucleotide sequence of its long terminal repeats.

Authors:  A A Bayev; N V Lyubomirskaya; E B Dzhumagaliev; E V Ananiev; I G Amiantova; Y V Ilyin
Journal:  Nucleic Acids Res       Date:  1984-04-25       Impact factor: 16.971

10.  Genes for tRNALys5 from Drosophila melanogaster.

Authors:  D DeFranco; K B Burke; S Hayashi; G M Tener; R C Miller; D Söll
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971
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