Literature DB >> 10438794

Purification and ligand binding of EmrR, a regulator of a multidrug transporter.

A Brooun1, J J Tomashek, K Lewis.   

Abstract

EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump-2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone-with affinities in the micromolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimeric EmrR.

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Year:  1999        PMID： 10438794      PMCID： PMC94011     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  20 in total

Review 1.  Multidrug resistance: Versatile drug sensors of bacterial cells.

Authors:  K Lewis
Journal:  Curr Biol       Date:  1999-06-03       Impact factor: 10.834

2.  Nucleotide sequence of the Escherichia coli regulatory gene mprA and construction and characterization of mprA-deficient mutants.

Authors:  I del Castillo; J E González-Pastor; J L San Millán; F Moreno
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator in Escherichia coli.

Authors:  R G Martin; K W Jair; R E Wolf; J L Rosner
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490

4.  The drug-binding activity of the multidrug-responding transcriptional regulator BmrR resides in its C-terminal domain.

Authors:  P N Markham; M Ahmed; A A Neyfakh
Journal:  J Bacteriol       Date:  1996-03       Impact factor: 3.490

5.  Characterization of MarR, the repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli.

Authors:  A S Seoane; S B Levy
Journal:  J Bacteriol       Date:  1995-06       Impact factor: 3.490

Review 6.  Multidrug resistance pumps in bacteria: variations on a theme.

Authors:  K Lewis
Journal:  Trends Biochem Sci       Date:  1994-03       Impact factor: 13.807

7.  A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates.

Authors:  M Ahmed; C M Borsch; S S Taylor; N Vázquez-Laslop; A A Neyfakh
Journal:  J Biol Chem       Date:  1994-11-11       Impact factor: 5.157

8.  EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrAB.

Authors:  O Lomovskaya; K Lewis; A Matin
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

9.  Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences.

Authors:  R G Martin; J L Rosner
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-06       Impact factor: 11.205

10.  Thiolactomycin resistance in Escherichia coli is associated with the multidrug resistance efflux pump encoded by emrAB.

Authors:  H Furukawa; J T Tsay; S Jackowski; Y Takamura; C O Rock
Journal:  J Bacteriol       Date:  1993-06       Impact factor: 3.490
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  31 in total

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Authors:  K Lewis
Journal:  Antimicrob Agents Chemother       Date:  2001-04       Impact factor: 5.191

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Authors:  M Putman; H W van Veen; W N Konings
Journal:  Microbiol Mol Biol Rev       Date:  2000-12       Impact factor: 11.056

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Authors:  A Xiong; A Gottman; C Park; M Baetens; S Pandza; A Matin
Journal:  Antimicrob Agents Chemother       Date:  2000-10       Impact factor: 5.191

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Authors:  M Ines Borges-Walmsley; Kenneth S McKeegan; Adrian R Walmsley
Journal:  Biochem J       Date:  2003-12-01       Impact factor: 3.857

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Authors:  Natalya Baranova; Hiroshi Nikaido
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

6.  LVIS553 transcriptional regulator specifically recognizes novobiocin as an effector molecule.

Authors:  Fernando A Pagliai; Christopher L Gardner; Santosh G Pande; Graciela L Lorca
Journal:  J Biol Chem       Date:  2010-03-22       Impact factor: 5.157

Review 7.  Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance.

Authors:  Lucía Fernández; Robert E W Hancock
Journal:  Clin Microbiol Rev       Date:  2012-10       Impact factor: 26.132

8.  Escherichia coli mar and acrAB mutants display no tolerance to simple alcohols.

Authors:  Jonas Ankarloo; Susanne Wikman; Ian A Nicholls
Journal:  Int J Mol Sci       Date:  2010-03-31       Impact factor: 5.923

9.  Structural and biochemical characterization of MepR, a multidrug binding transcription regulator of the Staphylococcus aureus multidrug efflux pump MepA.

Authors:  Muthiah Kumaraswami; Jason T Schuman; Susan M Seo; Glenn W Kaatz; Richard G Brennan
Journal:  Nucleic Acids Res       Date:  2009-01-07       Impact factor: 16.971

10.  Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials.

Authors:  Yilmaz Alguel; Cuixiang Meng; Wilson Terán; Tino Krell; Juan L Ramos; María-Trinidad Gallegos; Xiaodong Zhang
Journal:  J Mol Biol       Date:  2007-03-30       Impact factor: 5.469
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