Literature DB >> 10427741

Dilute spin-exchange assignment of solid-state NMR spectra of oriented proteins: acetylcholine M2 in bilayers.

F M Marassi1, J J Gesell, A P Valente, Y Kim, M Oblatt-Montal, M Montal, S J Opella.   

Abstract

The assignment of amide resonances in the two-dimensional PISEMA (Polarization Inversion with Spin Exchange at the Magic Angle) spectrum of uniformly 15N labeled M2 peptide corresponding to the channel-lining segment of the acetylcholine receptor in oriented phospholipid bilayers is described. The majority of the resonances were assigned through comparisons with spectra from selectively 15N labeled recombinant peptides and specifically 15N labeled synthetic peptides. Some resonances were assigned to specific amino acid residues by means of homonuclear 15N spin-exchange spectroscopy. A modification to the conventional spin-exchange pulse sequence that significantly shortens the length of the experiments by combining the intervals for 15N spin-exchange and 1H magnetization recovery is described.

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Year:  1999        PMID： 10427741      PMCID： PMC3282054          DOI： 10.1023/a:1008391823293

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  16 in total

1.  Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy.

Authors:  S J Opella; F M Marassi; J J Gesell; A P Valente; Y Kim; M Oblatt-Montal; M Montal
Journal:  Nat Struct Biol       Date:  1999-04

2.  Uncoupling of local field spectra in nuclear magnetic resonance: determination of atomic positions in solids.

Authors:  J S Waugh
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

3.  Chemical synthesis and characterization of peptides and oligomeric proteins designed to form transmembrane ion channels.

Authors:  T Iwamoto; A Grove; M O Montal; M Montal; J M Tomich
Journal:  Int J Pept Protein Res       Date:  1994-06

4.  High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR.

Authors:  R R Ketchem; W Hu; T A Cross
Journal:  Science       Date:  1993-09-10       Impact factor: 47.728

5.  Solid-state NMR studies of the membrane-bound closed state of the colicin E1 channel domain in lipid bilayers.

Authors:  Y Kim; K Valentine; S J Opella; S L Schendel; W A Cramer
Journal:  Protein Sci       Date:  1998-02       Impact factor: 6.725

Review 6.  From muscle endplate to brain synapses: a short history of synapses and agonist-activated ion channels.

Authors:  D Colquhoun; B Sakmann
Journal:  Neuron       Date:  1998-03       Impact factor: 17.173

Review 7.  Protein structure by solid-state NMR spectroscopy.

Authors:  S J Opella; P L Stewart; K G Valentine
Journal:  Q Rev Biophys       Date:  1987-02       Impact factor: 5.318

8.  Solid-state NMR triple-resonance backbone assignments in a protein.

Authors:  W M Tan; Z Gu; A C Zeri; S J Opella
Journal:  J Biomol NMR       Date:  1999-04       Impact factor: 2.835

9.  Three-dimensional solid-state NMR experiment that correlates the chemical shift and dipolar coupling frequencies of two heteronuclei.

Authors:  A Ramamoorthy; C H Wu; S J Opella
Journal:  J Magn Reson B       Date:  1995-04

Review 10.  NMR structural studies of membrane proteins.

Authors:  F M Marassi; S J Opella
Journal:  Curr Opin Struct Biol       Date:  1998-10       Impact factor: 6.809
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  21 in total

1.  Correlation of the structural and functional domains in the membrane protein Vpu from HIV-1.

Authors:  F M Marassi; C Ma; H Gratkowski; S K Straus; K Strebel; M Oblatt-Montal; M Montal; S J Opella
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-07       Impact factor: 11.205

2.  Orientation of cecropin A helices in phospholipid bilayers determined by solid-state NMR spectroscopy.

Authors:  F M Marassi; S J Opella; P Juvvadi; R B Merrifield
Journal:  Biophys J       Date:  1999-12       Impact factor: 4.033

3.  A solid-state NMR index of helical membrane protein structure and topology.

Authors:  F M Marassi; S J Opella
Journal:  J Magn Reson       Date:  2000-05       Impact factor: 2.229

4.  Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy.

Authors:  S J Opella; F M Marassi; J J Gesell; A P Valente; Y Kim; M Oblatt-Montal; M Montal
Journal:  Nat Struct Biol       Date:  1999-04

Review 5.  Nuclear magnetic resonance of membrane-associated peptides and proteins.

Authors:  S J Opella; C Ma; F M Marassi
Journal:  Methods Enzymol       Date:  2001       Impact factor: 1.600

Review 6.  Structure determination of membrane proteins by NMR spectroscopy.

Authors:  Stanley J Opella; Francesca M Marassi
Journal:  Chem Rev       Date:  2004-08       Impact factor: 60.622

7.  A model of the closed form of the nicotinic acetylcholine receptor m2 channel pore.

Authors:  Sanguk Kim; Aaron K Chamberlain; James U Bowie
Journal:  Biophys J       Date:  2004-08       Impact factor: 4.033

8.  Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers.

Authors:  Kaustubh R Mote; T Gopinath; Nathaniel J Traaseth; Jason Kitchen; Peter L Gor'kov; William W Brey; Gianluigi Veglia
Journal:  J Biomol NMR       Date:  2011-11       Impact factor: 2.835

9.  A resonance assignment method for oriented-sample solid-state NMR of proteins.

Authors:  Robert W Knox; George J Lu; Stanley J Opella; Alexander A Nevzorov
Journal:  J Am Chem Soc       Date:  2010-06-23       Impact factor: 15.419

10.  Experiments optimized for magic angle spinning and oriented sample solid-state NMR of proteins.

Authors:  Bibhuti B Das; Eugene C Lin; Stanley J Opella
Journal:  J Phys Chem B       Date:  2013-10-07       Impact factor: 2.991
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