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Literature DB >> 10359775

Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

Abstract

Membrane potential around single molecules has been measured by using the nonlinear optical phenomenon of second harmonic generation. This advance results from the interaction between a highly dipolar molecule with a selectively directed highly polarizable 1-nm gold particle. With this approach, a second harmonic signal, which is enhanced by the nanoparticle, is detected from a volume of nanometric dimensions. This present work clearly shows that functional cellular imaging around single molecules is possible by selectively directing an antibody with a 1-nm gold label to a specific membrane protein. The results of this work open the way for three-dimensional, high resolution functional imaging of membrane electrophysiology in cells and cellular networks.

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Year: 1999 PMID： 10359775 PMCID： PMC21978 DOI： 10.1073/pnas.96.12.6700

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Pool of ligand-bound platelet-derived growth factor beta-receptors remain activated and tyrosine phosphorylated after internalization.

Authors: A Sorkin; A Eriksson; C H Heldin; B Westermark; L Claesson-Welsh
Journal: J Cell Physiol Date: 1993-08 Impact factor: 6.384

2. Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment.

Authors: K M Berland; P T So; E Gratton
Journal: Biophys J Date: 1995-02 Impact factor: 4.033

3. Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range.

Authors: S W Hell; M Schrader; H T van der Voort
Journal: J Microsc Date: 1997-07 Impact factor: 1.758

4. Two-photon laser scanning fluorescence microscopy.

Authors: W Denk; J H Strickler; W W Webb
Journal: Science Date: 1990-04-06 Impact factor: 47.728

5. Nonlinear optical properties of potential sensitive styryl dyes.

Authors: J Y Huang; A Lewis; L Loew
Journal: Biophys J Date: 1988-05 Impact factor: 4.033

6. Probing membrane potential with nonlinear optics.

Authors: O Bouevitch; A Lewis; I Pinevsky; J P Wuskell; L M Loew
Journal: Biophys J Date: 1993-08 Impact factor: 4.033

7. Direct measurement of coupling between dendritic spines and shafts.

Authors: K Svoboda; D W Tank; W Denk
Journal: Science Date: 1996-05-03 Impact factor: 47.728

8. Charge shift optical probes of membrane potential. Theory.

Authors: L M Loew; G W Bonneville; J Surow
Journal: Biochemistry Date: 1978-09-19 Impact factor: 3.162

9. Cell surface changes accompanying the neural differentiation of an embryonal carcinoma cell line.

Authors: J M Levine; P Flynn
Journal: J Neurosci Date: 1986-11 Impact factor: 6.167

10. A comparison of the second harmonic generation from light-adapted, dark-adapted, blue, and acid purple membrane.

Authors: Z Chen; M Sheves; A Lewis; O Bouevitch
Journal: Biophys J Date: 1994-09 Impact factor: 4.033

17 in total

1. High-resolution nonlinear optical imaging of live cells by second harmonic generation.

Authors: P J Campagnola; M D Wei; A Lewis; L M Loew
Journal: Biophys J Date: 1999-12 Impact factor: 4.033

2. Coherent scattering in multi-harmonic light microscopy.

Authors: L Moreaux; O Sandre; S Charpak; M Blanchard-Desce; J Mertz
Journal: Biophys J Date: 2001-03 Impact factor: 4.033

3. Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.

Authors: Paul J Campagnola; Andrew C Millard; Mark Terasaki; Pamela E Hoppe; Christian J Malone; William A Mohler
Journal: Biophys J Date: 2002-01 Impact factor: 4.033

Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

1. Pool of ligand-bound platelet-derived growth factor beta-receptors remain activated and tyrosine phosphorylated after internalization.

2. Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment.

3. Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range.

4. Two-photon laser scanning fluorescence microscopy.

5. Nonlinear optical properties of potential sensitive styryl dyes.

6. Probing membrane potential with nonlinear optics.

7. Direct measurement of coupling between dendritic spines and shafts.

8. Charge shift optical probes of membrane potential. Theory.

9. Cell surface changes accompanying the neural differentiation of an embryonal carcinoma cell line.

10. A comparison of the second harmonic generation from light-adapted, dark-adapted, blue, and acid purple membrane.

1. High-resolution nonlinear optical imaging of live cells by second harmonic generation.

2. Coherent scattering in multi-harmonic light microscopy.

3. Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.

4. Sensitivity of second harmonic generation from styryl dyes to transmembrane potential.

5. Studies of chi(2)/chi(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy.

6. Wavelength- and time-dependence of potentiometric non-linear optical signals from styryl dyes.

Review 7. The role of synaptic ion channels in synaptic plasticity.

Review 8. Two-photon microscopy: shedding light on the chemistry of vision.

9. Lens-less surface second harmonic imaging.

Review 10. Applications of gold nanoparticles in cancer nanotechnology.