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Literature DB >> 9263436

Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range.

S W Hell¹, M Schrader, H T van der Voort.

Abstract

We report three-dimensional (3D) microscopy with nearly isotropic resolution in the lambda/5-lambda/10 range. Our approach combines 4PI-confocal two-photon fluorescence microscopy with image restoration. The 3D resolution is demonstrated with densely clustered beads as well as with F-actin fibers in mouse fibroblast cells. A comparison with unrestored two-photon confocal images reveals a total reduction of the uncertainty volume up to a factor of 15.

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Year: 1997 PMID： 9263436 DOI： 10.1046/j.1365-2818.1997.2410797.x

Source DB: PubMed Journal: J Microsc ISSN： 0022-2720 Impact factor: 1.758

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Cited

19 in total

1. High-resolution nonlinear optical imaging of live cells by second harmonic generation.

Authors: P J Campagnola; M D Wei; A Lewis; L M Loew
Journal: Biophys J Date: 1999-12 Impact factor: 4.033

2. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.

Authors: T A Klar; S Jakobs; M Dyba; A Egner; S W Hell
Journal: Proc Natl Acad Sci U S A Date: 2000-07-18 Impact factor: 11.205

3. Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

Authors: G Peleg; A Lewis; M Linial; L M Loew
Journal: Proc Natl Acad Sci U S A Date: 1999-06-08 Impact factor: 11.205

4. The connection between chromatin motion on the 100 nm length scale and core histone dynamics in live XTC-2 cells and isolated nuclei.

Authors: Sara K Davis; Christopher J Bardeen
Journal: Biophys J Date: 2004-01 Impact factor: 4.033

Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range.

1. High-resolution nonlinear optical imaging of live cells by second harmonic generation.

2. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.

3. Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

4. The connection between chromatin motion on the 100 nm length scale and core histone dynamics in live XTC-2 cells and isolated nuclei.

5. Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy.

6. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.

7. Microscopy and its focal switch.

8. 4Pi-confocal imaging in fixed biological specimens.

9. Electron tomography of ice-embedded prokaryotic cells.

10. Imaging nanometre-scale structure in cells using in situ aberration correction.