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Literature DB >> 8218895

Probing membrane potential with nonlinear optics.

O Bouevitch¹, A Lewis, I Pinevsky, J P Wuskell, L M Loew.

Abstract

The nonlinear optical phenomenon of second harmonic generation is shown to have intrinsic sensitivity to the voltage across a biological membrane. Our results demonstrate that this second order nonlinear optical process can be used to monitor membrane voltage with excellent signal to noise and other crucial advantages. These advantages suggest extensive use of this novel approach as an important new tool in elucidating membrane potential changes in biological systems. For this first demonstration of the effect we use a chiral styryl dye which exhibits gigantic second harmonic signals. Possible mechanisms of the voltage dependence of the second harmonic signal are discussed.

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Year: 1993 PMID： 8218895 PMCID： PMC1225769 DOI： 10.1016/S0006-3495(93)81126-3

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

10 in total

1. Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon.

Authors: I Freund; M Deutsch; A Sprecher
Journal: Biophys J Date: 1986-10 Impact factor: 4.033

2. Nonlinear optical properties of potential sensitive styryl dyes.

Authors: J Y Huang; A Lewis; L Loew
Journal: Biophys J Date: 1988-05 Impact factor: 4.033

3. Changes in axon fluorescence during activity: molecular probes of membrane potential.

Authors: L B Cohen; B M Salzberg; H V Davila; W N Ross; D Landowne; A S Waggoner; C H Wang
Journal: J Membr Biol Date: 1974 Impact factor: 1.843

4. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles.

Authors: P J Sims; A S Waggoner; C H Wang; J F Hoffman
Journal: Biochemistry Date: 1974-07-30 Impact factor: 3.162

5. Charge shift optical probes of membrane potential. Theory.

Authors: L M Loew; G W Bonneville; J Surow
Journal: Biochemistry Date: 1978-09-19 Impact factor: 3.162

6. Optical second-harmonic scattering in rat-tail tendon.

Authors: S Roth; I Freund
Journal: Biopolymers Date: 1981-06 Impact factor: 2.505

7. Mechanism of the membrane potential sensitivity of the fluorescent membrane probe merocyanine 540.

Authors: P R Dragsten; W W Webb
Journal: Biochemistry Date: 1978-11-28 Impact factor: 3.162

8. Evidence for a charge-shift electrochromic mechanism in a probe of membrane potential.

Authors: L M Loew; S Scully; L Simpson; A S Waggoner
Journal: Nature Date: 1979-10-11 Impact factor: 49.962

9. Spectra, membrane binding, and potentiometric responses of new charge shift probes.

Authors: E Fluhler; V G Burnham; L M Loew
Journal: Biochemistry Date: 1985-10-08 Impact factor: 3.162

10. Determination of the absolute orientation of the retinylidene chromophore in purple membrane by a second-harmonic interference technique.

Authors: J Y Huang; A Lewis
Journal: Biophys J Date: 1989-05 Impact factor: 4.033

10 in total

25 in total

10. Overcoming photodamage in second-harmonic generation microscopy: real-time optical recording of neuronal action potentials.

Authors: L Sacconi; D A Dombeck; W W Webb
Journal: Proc Natl Acad Sci U S A Date: 2006-02-17 Impact factor: 11.205

Probing membrane potential with nonlinear optics.

1. Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon.

2. Nonlinear optical properties of potential sensitive styryl dyes.

3. Changes in axon fluorescence during activity: molecular probes of membrane potential.

4. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles.

5. Charge shift optical probes of membrane potential. Theory.

6. Optical second-harmonic scattering in rat-tail tendon.

7. Mechanism of the membrane potential sensitivity of the fluorescent membrane probe merocyanine 540.

8. Evidence for a charge-shift electrochromic mechanism in a probe of membrane potential.

9. Spectra, membrane binding, and potentiometric responses of new charge shift probes.

10. Determination of the absolute orientation of the retinylidene chromophore in purple membrane by a second-harmonic interference technique.

1. High-resolution nonlinear optical imaging of live cells by second harmonic generation.

2. Coherent scattering in multi-harmonic light microscopy.

3. Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

4. Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy.

5. Sensitivity of second harmonic generation from styryl dyes to transmembrane potential.

6. Second-harmonic generation imaging of membrane potential with retinal analogues.

Review 7. Molecular contrast optical coherence tomography: a review.

8. Wavelength- and time-dependence of potentiometric non-linear optical signals from styryl dyes.

Review 9. Two-photon microscopy: shedding light on the chemistry of vision.

10. Overcoming photodamage in second-harmonic generation microscopy: real-time optical recording of neuronal action potentials.