| Literature DB >> 11222317 |
L Moreaux1, O Sandre, S Charpak, M Blanchard-Desce, J Mertz.
Abstract
By focusing a pulsed laser beam into a sample, harmonic up-conversion can be generated as well as multi-photon excited fluorescence. Whereas multi-photon excited fluorescence microscopy is well established, the use of multi-harmonic generation for three-dimensional image contrast is very recent. Both techniques can provide similar resolution and, for adequate radiating source density, comparable signal levels, allowing them to be combined in a single versatile instrument. However, harmonic generation differs fundamentally from fluorescence generation in that it is coherent and produces radiation patterns that are highly sensitive to phase. As such, multi-harmonic generation microscopy provides a unique window into molecular spatial organization that is inaccessible to fluorescence.Mesh:
Substances:
Year: 2001 PMID: 11222317 PMCID: PMC1301348 DOI: 10.1016/S0006-3495(01)76129-2
Source DB: PubMed Journal: Biophys J ISSN: 0006-3495 Impact factor: 4.033