Literature DB >> 10216295

A method to produce microseed stock for use in the crystallization of biological macromolecules.

J R Luft1, G T DeTitta.   

Abstract

A method is presented for producing a seed-stock mixture for macromolecular crystallization. A PTFE bead and micro-centrifuge tube act as mortar and pestle for pulverizing seed crystals of macromolecules. Energy for the bead's motion is supplied by a vortex mixer or an ultrasonic bath. The crushed crystal is serially diluted to prepare a seed-stock mixture of the desired concentration for crystallization. Crystals produced using both hanging-drop vapor diffusion and a capillary microbatch method show expected dilution behavior. This technique of producing seed stock is compared with traditional means and advantages over the standard protocol are demonstrated.

Mesh：

Substances：

Year:  1999        PMID： 10216295     DOI： 10.1107/s0907444999002085

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  32 in total

1.  Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme.

Authors:  Len Ito; Kentaro Shiraki; Hiroshi Yamaguchi
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-05-27

2.  Comparative analysis of amino acids and amino-acid derivatives in protein crystallization.

Authors:  Len Ito; Kentaro Shiraki; Hiroshi Yamaguchi
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-05-27

3.  Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a major fragment of the resuscitation-promoting factor RpfB from Mycobacterium tuberculosis.

Authors:  Alessia Ruggiero; Flavia Squeglia; Luciano Pirone; Stefania Correale; Rita Berisio
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-12-24

4.  Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.

Authors:  Cory J Gerdts; Valentina Tereshko; Maneesh K Yadav; Irina Dementieva; Frank Collart; Andrzej Joachimiak; Raymond C Stevens; Peter Kuhn; Anthony Kossiakoff; Rustem F Ismagilov
Journal:  Angew Chem Int Ed Engl       Date:  2006-12-11       Impact factor: 15.336

5.  Semi-automated microseeding of nanolitre crystallization experiments.

Authors:  Thomas S Walter; Erika J Mancini; Jan Kadlec; Stephen C Graham; René Assenberg; Jingshan Ren; Sarah Sainsbury; Raymond J Owens; David I Stuart; Jonathan M Grimes; Karl Harlos
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-12-20

6.  Phoenito experiments: combining the strengths of commercial crystallization automation.

Authors:  Janet Newman; Tam M Pham; Thomas S Peat
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-10-31

7.  Structural basis for the recognition of the scaffold protein Frmpd4/Preso1 by the TPR domain of the adaptor protein LGN.

Authors:  Hiroki Takayanagi; Satoru Yuzawa; Hideki Sumimoto
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-01-28       Impact factor: 1.056

8.  Crystallization and preliminary X-ray analysis of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Staphylococcus aureus.

Authors:  Sandeep Chhabra; Janet Newman; Thomas S Peat; Ross T Fernley; Joanne Caine; Jamie S Simpson; James D Swarbrick
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-04-30

9.  Entamoeba histolytica Rho1 regulates actin polymerization through a divergent, diaphanous-related formin.

Authors:  Dustin E Bosch; Bing Yang; David P Siderovski
Journal:  Biochemistry       Date:  2012-10-23       Impact factor: 3.162

10.  Crystal structure of an Fe-S cluster-containing fumarate hydratase enzyme from Leishmania major reveals a unique protein fold.

Authors:  Patricia R Feliciano; Catherine L Drennan; M Cristina Nonato
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-15       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.