Literature DB >> 10103282

Different strategies for molecular differentiation of Mycobacterium bovis strains isolated in Sardinia, Italy.

L A Sechi1, G Leori, S A Lollai, I Duprè, P Molicotti, G Fadda, S Zanetti.   

Abstract

Different genetic markers were used to analyze 22 Mycobacterium bovis strains isolated from cattle in Sardinia and one human isolate. IS6110 DNA fingerprinting differentiated the strains into six patterns, whereas with enterobacterial repetitive consensus sequence primers produced seven clusters. PCR ribotyping followed by digestion with HaeIII and PvuII produced five and seven patterns, respectively. PCR with the (GTG)5 oligonucleotide primer showed the best discriminatory power, generating eight clusters among the strains analyzed.

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Year:  1999        PMID： 10103282      PMCID： PMC91252     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

1.  Evaluation of strategies for molecular fingerprinting for use in the routine work of a Mycobacterium reference unit.

Authors:  S M Wilson; S Goss; F Drobniewski
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

2.  The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain.

Authors:  E Liébana; A Aranaz; L Dominguez; A Mateos; O González-Llamazares; E F Rodriguez-Ferri; M Domingo; D Vidal; D Cousins
Journal:  Vet Microbiol       Date:  1997-03       Impact factor: 3.293

3.  PCR-based fingerprinting of Mycobacterium bovis isolates.

Authors:  M Glennon; B Jäger; D Dowdall; M Maher; M Dawson; F Quigley; E Costello; T Smith
Journal:  Vet Microbiol       Date:  1997-03       Impact factor: 3.293

4.  Restriction fragment length polymorphism analysis of Mycobacterium bovis isolates from captive and free-ranging animals.

Authors:  D L Whipple; P R Clarke; J L Jarnagin; J B Payeur
Journal:  J Vet Diagn Invest       Date:  1997-10       Impact factor: 1.279

5.  Enterobacterial repetitive intergenic consensus sequences as molecular targets for typing of Mycobacterium tuberculosis strains.

Authors:  L A Sechi; S Zanetti; I Dupré; G Delogu; G Fadda
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

6.  Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis.

Authors:  D Cousins; S Williams; E Liébana; A Aranaz; A Bunschoten; J Van Embden; T Ellis
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

7.  Identification by spoligotyping of a caprine genotype in Mycobacterium bovis strains causing human tuberculosis.

Authors:  M Gutiérrez; S Samper; M S Jiménez; J D van Embden; J F Marín; C Martín
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

8.  Specific differentiation between Mycobacterium bovis BCG and virulent strains of the Mycobacterium tuberculosis complex.

Authors:  J Magdalena; P Supply; C Locht
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

9.  Restriction fragment length polymorphism and spacer oligonucleotide typing: a comparative analysis of fingerprinting strategies for Mycobacterium bovis.

Authors:  A Aranaz; E Liébana; A Mateos; L Domínguez; D Cousins
Journal:  Vet Microbiol       Date:  1998-04-15       Impact factor: 3.293

10.  Spacer oligotyping of Mycobacterium bovis isolates compared to typing by restriction fragment length polymorphism using PGRS, DR and IS6110 probes.

Authors:  S Roring; D Brittain; A E Bunschoten; M S Hughes; R A Skuce; J D van Embden; S D Neill
Journal:  Vet Microbiol       Date:  1998-03-15       Impact factor: 3.293
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  2 in total

1.  Molecular basis of rifampin and isoniazid resistance in Mycobacterium bovis strains isolated in Sardinia, Italy.

Authors:  L A Sechi; S Zanetti; M Sanguinetti; P Molicotti; L Romano; G Leori; G Delogu; S Boccia; M La Sorda; G Fadda
Journal:  Antimicrob Agents Chemother       Date:  2001-06       Impact factor: 5.191

2.  Distribution of a specific 500-base-pair fragment in mycobacterium bovis isolates from Sardinian cattle.

Authors:  L A Sechi; I Duprè; G Leori; G Fadda; S Zanetti
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948
  2 in total

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