Literature DB >> 9100324

The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain.

E Liébana1, A Aranaz, L Dominguez, A Mateos, O González-Llamazares, E F Rodriguez-Ferri, M Domingo, D Vidal, D Cousins.   

Abstract

A total of 129 Mycobacterium bovis strains from 5 different Spanish locations were fingerprinted using the IS6110 repetitive element. We demonstrated the presence of multiple copies (from 2 to 13) of IS6110 in a large proportion (47.4%) of the M.bovis strains isolated from cattle and we showed that these strains can be successfully differentiated by means of the RFLP with IS6110. All of the M. bovis strains isolated from goats had multiple copies of IS6110 and 4 bands of 2, 1.7, 1.4 and 1.3 kb were common in all the caprine RFLP patterns. The caprine strains formed a clearly separate cluster from the bovine strains.

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Year:  1997        PMID： 9100324     DOI： 10.1016/s0378-1135(96)01282-5

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  9 in total

1.  Study of restriction fragment length polymorphism analysis and spoligotyping for epidemiological investigation of Mycobacterium bovis infection.

Authors:  E Costello; D O'Grady; O Flynn; R O'Brien; M Rogers; F Quigley; J Egan; J Griffin
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

2.  Different strategies for molecular differentiation of Mycobacterium bovis strains isolated in Sardinia, Italy.

Authors:  L A Sechi; G Leori; S A Lollai; I Duprè; P Molicotti; G Fadda; S Zanetti
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

3.  Monitoring of transmission of tuberculosis between wild boars and cattle: genotypical analysis of strains by molecular epidemiology techniques.

Authors:  A Serraino; G Marchetti; V Sanguinetti; M C Rossi; R G Zanoni; L Catozzi; A Bandera; W Dini; W Mignone; F Franzetti; A Gori
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

4.  Identification of a novel DNA probe for strain typing Mycobacterium bovis by restriction fragment length polymorphism analysis.

Authors:  R O'Brien; O Flynn; E Costello; D O'Grady; M Rogers
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

5.  Characterization of the Mycobacterium bovis restriction fragment length polymorphism DNA probe pUCD and performance comparison with standard methods.

Authors:  R O'Brien; B S Danilowicz; L Bailey; O Flynn; E Costello; D O'Grady; M Rogers
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

6.  Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis.

Authors:  D Cousins; S Williams; E Liébana; A Aranaz; A Bunschoten; J Van Embden; T Ellis
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

7.  Distribution of a specific 500-base-pair fragment in mycobacterium bovis isolates from Sardinian cattle.

Authors:  L A Sechi; I Duprè; G Leori; G Fadda; S Zanetti
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

8.  Identification by spoligotyping of a caprine genotype in Mycobacterium bovis strains causing human tuberculosis.

Authors:  M Gutiérrez; S Samper; M S Jiménez; J D van Embden; J F Marín; C Martín
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

9.  Usefulness of spoligotyping in molecular epidemiology of Mycobacterium bovis-related infections in South America.

Authors:  M J Zumárraga; C Martin; S Samper; A Alito; O Latini; F Bigi; E Roxo; M E Cicuta; F Errico; M C Ramos; A Cataldi; D van Soolingen; M I Romano
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948
  9 in total

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