Literature DB >> 10022352

Strategy for membrane protein crystallization exemplified with OmpA and OmpX.

A Pautsch1, J Vogt, K Model, C Siebold, G E Schulz.   

Abstract

The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable for a broader range of membrane proteins.

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Year:  1999        PMID： 10022352

Source DB:  PubMed          Journal:  Proteins        ISSN： 0887-3585


  26 in total

1.  The C-terminal domain of the Bordetella pertussis autotransporter BrkA forms a pore in lipid bilayer membranes.

Authors:  J L Shannon; R C Fernandez
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  Crystal structure and catalytic mechanism of the LPS 3-O-deacylase PagL from Pseudomonas aeruginosa.

Authors:  Lucy Rutten; Jeroen Geurtsen; Wietske Lambert; Jeroen J M Smolenaers; Alexandre M Bonvin; Alex de Haan; Peter van der Ley; Maarten R Egmond; Piet Gros; Jan Tommassen
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-21       Impact factor: 11.205

3.  Reconstitution and alignment by a magnetic field of a beta-barrel membrane protein in bicelles.

Authors:  Mohamed N Triba; Manuela Zoonens; Jean-Luc Popot; Philippe F Devaux; Dror E Warschawski
Journal:  Eur Biophys J       Date:  2005-09-27       Impact factor: 1.733

4.  Magic-angle spinning solid-state NMR of a 144 kDa membrane protein complex: E. coli cytochrome bo3 oxidase.

Authors:  Heather L Frericks; Donghua H Zhou; Lai Lai Yap; Robert B Gennis; Chad M Rienstra
Journal:  J Biomol NMR       Date:  2006-09-09       Impact factor: 2.835

5.  An unexpected outcome of surface engineering an integral membrane protein: improved crystallization of cytochrome ba(3) from Thermus thermophilus.

Authors:  Bin Liu; V Mitch Luna; Ying Chen; C David Stout; James A Fee
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-11-21

6.  Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE.

Authors:  Ke Xia; Marta Manning; Helai Hesham; Qishan Lin; Christopher Bystroff; Wilfredo Colón
Journal:  Proc Natl Acad Sci U S A       Date:  2007-10-23       Impact factor: 11.205

7.  New tetragonal form of reaction centers from Rhodobacter sphaeroides and the involvement of a manganese ion at a crystal contact point.

Authors:  G Uyeda; A Cámara-Artigas; J C Williams; J P Allen
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-07-08

8.  Mitochondrial outer membrane proteins assist Bid in Bax-mediated lipidic pore formation.

Authors:  Blanca Schafer; Joel Quispe; Vineet Choudhary; Jerry E Chipuk; Teddy G Ajero; Han Du; Roger Schneiter; Tomomi Kuwana
Journal:  Mol Biol Cell       Date:  2009-02-25       Impact factor: 4.138

9.  Beta-barrel proteins that reside in the Escherichia coli outer membrane in vivo demonstrate varied folding behavior in vitro.

Authors:  Nancy K Burgess; Thuy P Dao; Ann Marie Stanley; Karen G Fleming
Journal:  J Biol Chem       Date:  2008-07-19       Impact factor: 5.157

Review 10.  Small angle neutron scattering for the study of solubilised membrane proteins.

Authors:  Cécile Breyton; Frank Gabel; Mathilde Lethier; Ali Flayhan; Grégory Durand; Jean-Michel Jault; Céline Juillan-Binard; Lionel Imbert; Martine Moulin; Stéphanie Ravaud; Michael Härtlein; Christine Ebel
Journal:  Eur Phys J E Soft Matter       Date:  2013-07-16       Impact factor: 1.890
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