Literature DB >> 9889245

Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria.

A Klausegger1, M Hell, A Berger, K Zinober, S Baier, N Jones, W Sperl, B Kofler.   

Abstract

Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.

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Year:  1999        PMID： 9889245      PMCID： PMC84344     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Rapid detection of neonatal sepsis using polymerase chain reaction.

Authors:  N Laforgia; B Coppola; R Carbone; A Grassi; A Mautone; A Iolascon
Journal:  Acta Paediatr       Date:  1997-10       Impact factor: 2.299

2.  The detection of microbial DNA in the blood: a sensitive method for diagnosing bacteremia and/or bacterial translocation in surgical patients.

Authors:  T D Kane; J W Alexander; J A Johannigman
Journal:  Ann Surg       Date:  1998-01       Impact factor: 12.969

3.  Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.

Authors:  A Meier; D H Persing; M Finken; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

4.  Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing.

Authors:  D Goldenberger; A Künzli; P Vogt; R Zbinden; M Altwegg
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

5.  Detection of bacterial DNA in joint samples from patients with undifferentiated arthritis and reactive arthritis, using polymerase chain reaction with universal 16S ribosomal RNA primers.

Authors:  B Wilbrink; I M van der Heijden; L M Schouls; J D van Embden; J M Hazes; F C Breedveld; P P Tak
Journal:  Arthritis Rheum       Date:  1998-03

6.  Quantitation of bacteria in cerebrospinal fluid and blood of children with meningitis and its diagnostic significance.

Authors:  L J La Scolea; D Dryja
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

7.  Clinical impact of rapid in vitro susceptibility testing and bacterial identification.

Authors:  G V Doern; R Vautour; M Gaudet; B Levy
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

8.  PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid.

Authors:  K Greisen; M Loeffelholz; A Purohit; D Leong
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948
  8 in total
  25 in total

1.  Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.

Authors:  C E Corless; M Guiver; R Borrow; V Edwards-Jones; E B Kaczmarski; A J Fox
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

Review 2.  The role of DNA amplification technology in the diagnosis of infectious diseases.

Authors:  M Louie; L Louie; A E Simor
Journal:  CMAJ       Date:  2000-08-08       Impact factor: 8.262

3.  Real-time PCR for detection and differentiation of gram-positive and gram-negative bacteria.

Authors:  Sven Klaschik; Lutz E Lehmann; Ansgar Raadts; Malte Book; Andreas Hoeft; Frank Stuber
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

4.  Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.

Authors:  Sven Klaschik; Lutz E Lehmann; Ansgar Raadts; Andreas Hoeft; Frank Stuber
Journal:  Mol Biotechnol       Date:  2002-11       Impact factor: 2.695

5.  Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.

Authors:  Tamara Newsome; Bing-Jie Li; Nianxiang Zou; Shyh-Ching Lo
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

6.  Absence of Bacteria in the Temporal Arteries of Patients with Giant Cell Arteritis.

Authors:  Curry L Koening; Spencer G Peterson; Nicole L Podnecky; Herbert P Schweizer; Dean Y Li; Bradley J Katz
Journal:  J Clin Rheumatol       Date:  2016-01       Impact factor: 3.517

7.  Frequency of detection of bacterial DNA in whole blood of donors.

Authors:  N A Fedorov; A A Yolovl; E B Zhiburt; Yu S Sukhanov; A N Kruglov; A V Stonogin; S A Golosova; E G Cherkasov; M P Grishaev; E N Fedorov; A S Turgiev
Journal:  Dokl Biol Sci       Date:  2005 May-Jun

8.  An improved method of elimination of DNA from PCR reagents.

Authors:  Farjana B Rowther; Camilla Rodrigues; Ajita P Mehta; Minal S Deshmukh; Farhad N Kapadia; Ashit Hegde; Vinay R Joshi
Journal:  Mol Diagn       Date:  2005

9.  Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.

Authors:  Maneesh R Pingle; Kathleen Granger; Philip Feinberg; Rebecca Shatsky; Bram Sterling; Mark Rundell; Eric Spitzer; Davise Larone; Linnie Golightly; Francis Barany
Journal:  J Clin Microbiol       Date:  2007-04-11       Impact factor: 5.948

10.  An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications.

Authors:  Sophie Champlot; Camille Berthelot; Mélanie Pruvost; E Andrew Bennett; Thierry Grange; Eva-Maria Geigl
Journal:  PLoS One       Date:  2010-09-28       Impact factor: 3.240
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