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Literature DB >> 9889237

Baculovirus expression of chimeric hepatitis B virus core particles with hepatitis E virus epitopes and their use in a hepatitis E immunoassay.

A Touze¹, N Enogat, Y Buisson, P Coursaget.

Abstract

Two hepatitis B core proteins bearing the immunodominant region of the hepatitis E virus (HEV) capsid protein, one at the C terminus of hepatitis B virus core antigen (HBcAg) and the other within the HBcAg immunodominant loop, were constructed. Both chimeric proteins exhibited HEV reactivity, but only the first construct retained HBcAg reactivity. The second construct was used to develop an anti-HEV test which is equivalent to a commercial test for the detection of anti-HEV immunoglobulin G (IgG) but is more sensitive for the detection of anti-HEV IgM.

Entities: Disease Species

Mesh：

Substances：

Year: 1999 PMID： 9889237 PMCID： PMC84333

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

13 in total

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Authors: C Prodromou; L H Pearl
Journal: Protein Eng Date: 1992-12

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Authors: P Coursaget; P Maupas; A Goudeau; I Millman
Journal: J Immunol Methods Date: 1976 Impact factor: 2.303

3. Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides.

Authors: G J Dawson; K H Chau; C M Cabal; P O Yarbough; G R Reyes; I K Mushahwar
Journal: J Virol Methods Date: 1992-07 Impact factor: 2.014

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Authors: F Takemura; T Ishii; N Fujii; T Uchida
Journal: Nucleic Acids Res Date: 1990-08-11 Impact factor: 16.971

5. Hepatitis E virus: identification of type-common epitopes.

Authors: P O Yarbough; A W Tam; K E Fry; K Krawczynski; K A McCaustland; D W Bradley; G R Reyes
Journal: J Virol Date: 1991-11 Impact factor: 5.103

6. Insertions within the hepatitis B virus capsid protein influence capsid formation and RNA encapsidation.

Authors: B Beames; R E Lanford
Journal: J Virol Date: 1995-11 Impact factor: 5.103

7. Mapping of linear B cell epitopes on open reading frames 2- and 3-encoded proteins of hepatitis E virus using synthetic peptides.

Authors: P Coursaget; Y Buisson; N Depril; P le Cann; M Chabaud; C Molinié; R Roue
Journal: FEMS Microbiol Lett Date: 1993-05-15 Impact factor: 2.742

8. Hybrid hepatitis B virus nucleocapsid bearing an immunodominant region from hepatitis B virus surface antigen.

Authors: G Borisova; B Arya; A Dislers; O Borschukova; V Tsibinogin; D Skrastina; M A Eldarov; P Pumpens; K G Skryabin; E Grens
Journal: J Virol Date: 1993-06 Impact factor: 5.103

9. Three-dimensional structure of hepatitis B virus core particles determined by electron cryomicroscopy.

Authors: R A Crowther; N A Kiselev; B Böttcher; J A Berriman; G P Borisova; V Ose; P Pumpens
Journal: Cell Date: 1994-06-17 Impact factor: 41.582

10. Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.

Authors: A W Tam; M M Smith; M E Guerra; C C Huang; D W Bradley; K E Fry; G R Reyes
Journal: Virology Date: 1991-11 Impact factor: 3.616

6 in total

1. Detection of neutralizing antibodies against human papillomaviruses (HPV) by inhibition of gene transfer mediated by HPV pseudovirions.

Authors: Latifa Bousarghin; Alba-Lucia Combita-Rojas; Antoine Touzé; Slimane El Mehdaoui; Pierre-Yves Sizaret; Maria-Mercedes Bravo; Pierre Coursaget
Journal: J Clin Microbiol Date: 2002-03 Impact factor: 5.948

2. Quantitation of immunoglobulin to hepatitis E virus by enzyme immunoassay.

Authors: Bruce L Innis; Jitvimol Seriwatana; Robin A Robinson; Mrigendra P Shrestha; Patrice O Yarbough; Charles F Longer; Robert M Scott; David W Vaughn; Khin Saw Aye Myint
Journal: Clin Diagn Lab Immunol Date: 2002-05

3. Nasal immunization of mice with human papillomavirus type 16 (HPV-16) virus-like particles or with the HPV-16 L1 gene elicits specific cytotoxic T lymphocytes in vaginal draining lymph nodes.

Authors: C Dupuy; D Buzoni-Gatel; A Touzé; D Bout; P Coursaget
Journal: J Virol Date: 1999-11 Impact factor: 5.103