| Literature DB >> 1644893 |
G J Dawson1, K H Chau, C M Cabal, P O Yarbough, G R Reyes, I K Mushahwar.
Abstract
Four recombinant antigens representing two distinct antigenic domains from two different strains of hepatitis E virus (HEV), were used individually to develop four ELISAs designed to detect antibodies to HEV. Both IgG and IgM class antibodies to HEV were detected in 7 of 8 pedigreed serum/plasma from known outbreaks of HEV in Mexico, Burma, Somalia and Pakistan. In addition, specific HEV-antibodies were detected in cynomolgus macaques following inoculation with various HEV strains. Anti-HEV was also detected in 8 of 386 (2.1%) randomly selected American blood donors. Supplemental tests utilizing both synthetic peptides and specific blocking assays provided additional serologic data confirming the presence of anti-HEV. Similar prevalence studies on a limited number of available sera from other geographical regions (Alaska, Japan, Germany, New Zealand, Thailand and Mexico) confirmed the presence of anti-HEV in at least 1.1 to 7.6% of the specimens.Entities:
Mesh：
Substances：
Year: 1992 PMID： 1644893 DOI： 10.1016/0166-0934(92)90180-l
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014