Literature DB >> 9874762

A microfabricated device for sizing and sorting DNA molecules.

H P Chou1, C Spence, A Scherer, S Quake.   

Abstract

We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.

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Year:  1999        PMID： 9874762      PMCID： PMC15083          DOI： 10.1073/pnas.96.1.11

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  7 in total

1.  Sizing single DNA molecules.

Authors:  X H Guo; E J Huff; D C Schwartz
Journal:  Nature       Date:  1992-10-29       Impact factor: 49.962

2.  A new strategy for genome sequencing.

Authors:  J C Venter; H O Smith; L Hood
Journal:  Nature       Date:  1996-05-30       Impact factor: 49.962

3.  Integrated capillary electrophoresis on flexible silicone microdevices:  analysis of DNA restriction fragments and detection of single DNA molecules on microchips.

Authors:  C S Effenhauser; G J Bruin; A Paulus; M Ehrat
Journal:  Anal Chem       Date:  1997-09-01       Impact factor: 6.986

4.  High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping.

Authors:  W Cai; J Jing; B Irvin; L Ohler; E Rose; H Shizuya; U J Kim; M Simon; T Anantharaman; B Mishra; D C Schwartz
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-31       Impact factor: 11.205

5.  Large DNA fragment sizing by flow cytometry: application to the characterization of P1 artificial chromosome (PAC) clones.

Authors:  Z Huang; J T Petty; B O'Quinn; J L Longmire; N C Brown; J H Jett; R A Keller
Journal:  Nucleic Acids Res       Date:  1996-11-01       Impact factor: 16.971

6.  Patterned delivery of immunoglobulins to surfaces using microfluidic networks.

Authors:  E Delamarche; A Bernard; H Schmid; B Michel; H Biebuyck
Journal:  Science       Date:  1997-05-02       Impact factor: 47.728

7.  Fabrication of submicrometer features on curved substrates by microcontact printing.

Authors:  R J Jackman; J L Wilbur; G M Whitesides
Journal:  Science       Date:  1995-08-04       Impact factor: 47.728
  7 in total
  26 in total

1.  A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion.

Authors:  Carl L Hansen; Emmanuel Skordalakes; James M Berger; Stephen R Quake
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-16       Impact factor: 11.205

2.  Quantifying double-strand breaks and clustered damages in DNA by single-molecule laser fluorescence sizing.

Authors:  Elena M Filippova; Denise C Monteleone; John G Trunk; Betsy M Sutherland; Stephen R Quake; John C Sutherland
Journal:  Biophys J       Date:  2003-02       Impact factor: 4.033

3.  DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags.

Authors:  Eugene Y Chan; Nuno M Goncalves; Rebecca A Haeusler; Amie J Hatch; Jonathan W Larson; Anthony M Maletta; Gregory R Yantz; Eugene D Carstea; Martin Fuchs; Gordon G Wong; Steven R Gullans; Rudolf Gilmanshin
Journal:  Genome Res       Date:  2004-06       Impact factor: 9.043

4.  Single-file electrophoretic transport and counting of individual DNA molecules in surfactant nanotubes.

Authors:  Michal Tokarz; Björn Akerman; Jessica Olofsson; Jean-Francois Joanny; Paul Dommersnes; Owe Orwar
Journal:  Proc Natl Acad Sci U S A       Date:  2005-06-16       Impact factor: 11.205

5.  Space- and time-resolved spectrophotometry in microsystems.

Authors:  Nicolae Damean; Samuel K Sia; Vincent Linder; Max Narovlyansky; George M Whitesides
Journal:  Proc Natl Acad Sci U S A       Date:  2005-07-08       Impact factor: 11.205

6.  Conformation, length, and speed measurements of electrodynamically stretched DNA in nanochannels.

Authors:  Christian H Reccius; Samuel M Stavis; John T Mannion; Larry P Walker; H G Craighead
Journal:  Biophys J       Date:  2008-03-13       Impact factor: 4.033

7.  Cylindrical illumination confocal spectroscopy: rectifying the limitations of confocal single molecule spectroscopy through one-dimensional beam shaping.

Authors:  Kelvin J Liu; Tza-Huei Wang
Journal:  Biophys J       Date:  2008-05-30       Impact factor: 4.033

Review 8.  Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.

Authors:  Kevin D Dorfman; Scott B King; Daniel W Olson; Joel D P Thomas; Douglas R Tree
Journal:  Chem Rev       Date:  2012-11-12       Impact factor: 60.622

9.  Single molecule analysis of bacterial polymerase chain reaction products in submicrometer fluidic channels.

Authors:  Samuel M Stavis; Stéphane C Corgié; Benjamin R Cipriany; Harold G Craighead; Larry P Walker
Journal:  Biomicrofluidics       Date:  2007-09-20       Impact factor: 2.800

10.  A method for nanofluidic device prototyping using elastomeric collapse.

Authors:  Seung-min Park; Yun Suk Huh; Harold G Craighead; David Erickson
Journal:  Proc Natl Acad Sci U S A       Date:  2009-08-27       Impact factor: 11.205
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