Literature DB >> 9874676

Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay.

S L Schoenthaler1, S Kapil.   

Abstract

We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10(4) bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.

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Year:  1999        PMID： 9874676      PMCID： PMC95672          DOI： 10.1128/CDLI.6.1.130-132.1999

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  11 in total

1.  A review of evidence implicating bovine coronavirus in the etiology of winter dysentery in cows: an enigma resolved?

Authors:  L J Saif
Journal:  Cornell Vet       Date:  1990-10

2.  Evaluation of two antigen-capture ELISAs using polyclonal or monoclonal antibodies for the detection of bovine coronavirus.

Authors:  D R Smith; H Tsunemitsu; R A Heckert; L J Saif
Journal:  J Vet Diagn Invest       Date:  1996-01       Impact factor: 1.279

3.  Chronic shedding of bovine enteric coronavirus antigen-antibody complexes by clinically normal cows.

Authors:  C F Crouch; H Bielefeldt Ohmann; T C Watts; L A Babiuk
Journal:  J Gen Virol       Date:  1985-07       Impact factor: 3.891

4.  Detection of bovine coronavirus by enzyme-linked immunosorbent assay using monoclonal antibodies.

Authors:  M Sato; H Akashi
Journal:  J Vet Med Sci       Date:  1993-10       Impact factor: 1.267

5.  Monoclonal antibody capture enzyme-linked immunosorbent assay for detection of bovine enteric coronavirus.

Authors:  C F Crouch; T J Raybould; S D Acres
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

6.  Application of immunohistochemistry and in situ hybridization for detection of bovine coronavirus in paraffin-embedded, formalin-fixed intestines.

Authors:  Z Zhang; G A Andrews; C Chard-Bergstrom; H C Minocha; S Kapil
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

7.  Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: establishment of an efficient ELISA for antigen detection in feces.

Authors:  C P Czerny; W Eichhorn
Journal:  Vet Microbiol       Date:  1989-06       Impact factor: 3.293

8.  Evaluation of ELISA and electron microscopy for the detection of coronavirus and rotavirus in bovine faeces.

Authors:  D J Reynolds; D Chasey; A C Scott; J C Bridger
Journal:  Vet Rec       Date:  1984-04-21       Impact factor: 2.695

9.  Development of monoclonal antibody ELISA for simultaneous detection of bovine coronavirus, rotavirus serogroup A, and Escherichia coli K99 antigen in feces of calves.

Authors:  C J Thorns; M M Bell; D Chasey; J Chesham; P L Roeder
Journal:  Am J Vet Res       Date:  1992-01       Impact factor: 1.156

10.  Development of protein A-gold immunoelectron microscopy for detection of bovine coronavirus in calves: comparison with ELISA and direct immunofluorescence of nasal epithelial cells.

Authors:  R A Heckert; L J Saif; G W Myers
Journal:  Vet Microbiol       Date:  1989-03       Impact factor: 3.293
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  8 in total

1.  Development, characterization, and diagnostic applications of monoclonal antibodies against bovine rotavirus.

Authors:  Y Al-Yousif; F Al-Majhdi; C Chard-Bergstrom; J Anderson; S Kapil
Journal:  Clin Diagn Lab Immunol       Date:  2000-03

2.  Development of an antigen spot test for detection of coronavirus in bovine fecal samples.

Authors:  F Gaber; S Kapil
Journal:  Clin Diagn Lab Immunol       Date:  1999-07

3.  Development of a recombinant nucleoprotein-based enzyme-linked immunosorbent assay for quantification of antibodies against porcine reproductive and respiratory syndrome virus.

Authors:  S B Witte; C Chard-Bergstrom; T A Loughin; S Kapil
Journal:  Clin Diagn Lab Immunol       Date:  2000-07

4.  Bovine coronavirus associated syndromes.

Authors:  Mélanie J Boileau; Sanjay Kapil
Journal:  Vet Clin North Am Food Anim Pract       Date:  2010-03       Impact factor: 3.357

5.  Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus.

Authors:  Karen Miyuki Asano; Sibele Pinheiro de Souza; Iracema Nunes de Barros; Giselle Razera Ayres; Sheila Oliveira Souza Silva; Leonardo José Richtzenhain; Paulo E Brandão
Journal:  J Virol Methods       Date:  2010-08-17       Impact factor: 2.014

6.  Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia.

Authors:  M M Izzo; P D Kirkland; X Gu; Y Lele; A A Gunn; J K House
Journal:  Aust Vet J       Date:  2012-04       Impact factor: 1.281

7.  Improved detection of bovine coronavirus N gene in faeces of calves infected naturally by a semi-nested PCR assay and an internal control.

Authors:  Elisabete Takiuchi; Danilo T Stipp; Alice F Alfieri; Amauri A Alfieri
Journal:  J Virol Methods       Date:  2005-09-22       Impact factor: 2.014

8.  The use of bovine serum protein as an oral support therapy following coronavirus challenge in calves.

Authors:  J D Arthington; C A Jaynes; H D Tyler; S Kapil; J D Quigley
Journal:  J Dairy Sci       Date:  2002-05       Impact factor: 4.034
  8 in total

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