Literature DB >> 10882676

Development of a recombinant nucleoprotein-based enzyme-linked immunosorbent assay for quantification of antibodies against porcine reproductive and respiratory syndrome virus.

S B Witte1, C Chard-Bergstrom, T A Loughin, S Kapil.   

Abstract

A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

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Year:  2000        PMID： 10882676      PMCID： PMC95938          DOI： 10.1128/CDLI.7.4.700-702.2000

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  17 in total

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2.  General method for direct cloning of DNA fragments generated by the polymerase chain reaction.

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4.  Assessment of seropositivity to porcine reproductive and respiratory syndrome (PRRS) virus in swine herds in Ontario--1978 to 1982.

Authors:  S Carman; S E Sanford; S Dea
Journal:  Can Vet J       Date:  1995-12       Impact factor: 1.008

Review 5.  Diagnosis of PRRS.

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Journal:  Vet Microbiol       Date:  1997-04       Impact factor: 3.293

6.  Preparative and analytical purification of DNA from agarose.

Authors:  B Vogelstein; D Gillespie
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7.  An ELISA for porcine reproductive and respiratory syndrome: production of antigen of high quality.

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Authors:  E A Nelson; J Christopher-Hennings; T Drew; G Wensvoort; J E Collins; D A Benfield
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

9.  Bovine viral diarrhea virus isolated from fetal calf serum enhances pathogenicity of attenuated transmissible gastroenteritis virus in neonatal pigs.

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Authors:  H S Kim; J Kwang; I J Yoon; H S Joo; M L Frey
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574
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  6 in total

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Journal:  Clin Diagn Lab Immunol       Date:  2000-07

3.  Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China.

Authors:  Xiaofang Hao; Zengjun Lu; Wendong Kuang; Pu Sun; Yu Fu; Lei Wu; Qing Zhao; Huifang Bao; Yuanfang Fu; Yimei Cao; Pinghua Li; Xingwen Bai; Dong Li; Zaixin Liu
Journal:  Virol J       Date:  2011-02-19       Impact factor: 4.099

4.  Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus.

Authors:  Neal H Ferrin; Ying Fang; Craig R Johnson; Michael P Murtaugh; Dale D Polson; Montserrat Torremorell; Marie L Gramer; Eric A Nelson
Journal:  Clin Diagn Lab Immunol       Date:  2004-05

5.  Detection of asymptomatic antigenemia in pigs infected by porcine reproductive and respiratory syndrome virus (PRRSV) by a novel capture immunoassay with monoclonal antibodies against the nucleocapsid protein of PRRSV.

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6.  Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis.

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  6 in total

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