Literature DB >> 9872402

Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris.

U Hochstrasser1, M Lüscher, C De Virgilio, T Boller, A Wiemken.   

Abstract

The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZ alphaC, containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory pathway. Transformed Pichia produced and secreted a functional 6-SFT which had characteristics similar to the barley enzyme, but had a pronounced additional 1-SST activity when incubated with sucrose.

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Year:  1998        PMID： 9872402     DOI： 10.1016/s0014-5793(98)01487-2

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  13 in total

1.  Expression and Activity Analysis of Fructosyltransferase from Aspergillus oryzae.

Authors:  Lihong Guan; Liping Chen; Yongsen Chen; Nu Zhang; Yawei Han
Journal:  Protein J       Date:  2017-08       Impact factor: 2.371

2.  Distinct regulation of sucrose: sucrose-1-fructosyltransferase (1-SST) and sucrose: fructan-6-fructosyltransferase (6-SFT), the key enzymes of fructan synthesis in barley leaves: 1-SST as the pacemaker.

Authors:  Vinay J Nagaraj; Denise Altenbach; Virginie Galati; Marcel Lüscher; Alain D Meyer; Thomas Boller; Andres Wiemken
Journal:  New Phytol       Date:  2004-01-14       Impact factor: 10.151

3.  Gene cloning and functional characterization by heterologous expression of the fructosyltransferase of Aspergillus sydowi IAM 2544.

Authors:  A G Heyer; R Wendenburg
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

4.  Cloning and functional analysis of sucrose:sucrose 1-fructosyltransferase from tall fescue.

Authors:  M Lüscher; U Hochstrasser; G Vogel; R Aeschbacher; V Galati; C J Nelson; T Boller; A Wiemken
Journal:  Plant Physiol       Date:  2000-11       Impact factor: 8.340

5.  Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat.

Authors:  Akira Kawakami; Midori Yoshida
Journal:  Planta       Date:  2005-07-21       Impact factor: 4.116

6.  Transforming a fructan:fructan 6G-fructosyltransferase from perennial ryegrass into a sucrose:sucrose 1-fructosyltransferase.

Authors:  Bertrand Lasseur; Lindsey Schroeven; Willem Lammens; Katrien Le Roy; German Spangenberg; Hélène Manduzio; Rudy Vergauwen; Jérémy Lothier; Marie-Pascale Prud'homme; Wim Van den Ende
Journal:  Plant Physiol       Date:  2008-10-24       Impact factor: 8.340

Review 7.  Recombinant protein expression in Pichia pastoris.

Authors:  J M Cregg; J L Cereghino; J Shi; D R Higgins
Journal:  Mol Biotechnol       Date:  2000-09       Impact factor: 2.860

8.  Cloning and functional analysis of a fructosyltransferase cDNA for synthesis of highly polymerized levans in timothy (Phleum pratense L.).

Authors:  Ken-ichi Tamura; Akira Kawakami; Yasuharu Sanada; Kazuhiro Tase; Toshinori Komatsu; Midori Yoshida
Journal:  J Exp Bot       Date:  2009       Impact factor: 6.992

Review 9.  Fructan and its relationship to abiotic stress tolerance in plants.

Authors:  David P Livingston; Dirk K Hincha; Arnd G Heyer
Journal:  Cell Mol Life Sci       Date:  2009-03-17       Impact factor: 9.261

10.  An acceptor-substrate binding site determining glycosyl transfer emerges from mutant analysis of a plant vacuolar invertase and a fructosyltransferase.

Authors:  Denise Altenbach; Enrique Rudiño-Pinera; Clarita Olvera; Thomas Boller; Andres Wiemken; Tita Ritsema
Journal:  Plant Mol Biol       Date:  2008-09-28       Impact factor: 4.076
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