Literature DB >> 11098467

Recombinant protein expression in Pichia pastoris.

J M Cregg1, J L Cereghino, J Shi, D R Higgins.   

Abstract

The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool. What began more than 20 years ago as a program to convert abundant methanol to a protein source for animal feed has been developed into what is today two important biological tools: a model eukaryote used in cell biology research and a recombinant protein production system. To date well over 200 heterologous proteins have been expressed in P. pastoris. Significant advances in the development of new strains and vectors, improved techniques, and the commercial availability of these tools coupled with a better understanding of the biology of Pichia species have led to this microbe's value and power in commercial and research labs alike.

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Year:  2000        PMID： 11098467     DOI： 10.1385/MB:16:1:23

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.860


  290 in total

1.  Production of synthetic spider dragline silk protein in Pichia pastoris.

Authors:  S R Fahnestock; L A Bedzyk
Journal:  Appl Microbiol Biotechnol       Date:  1997-01       Impact factor: 4.813

2.  Complete determination of disulfide forms of purified recombinant human serum albumin, secreted by the yeast Pichia pastoris.

Authors:  K Ikegaya; M Hirose; T Ohmura; K Nokihara
Journal:  Anal Chem       Date:  1997-06-01       Impact factor: 6.986

3.  Identification in vitro of a post-translational regulatory site in the hinge 1 region of Arabidopsis nitrate reductase.

Authors:  W Su; S C Huber; N M Crawford
Journal:  Plant Cell       Date:  1996-03       Impact factor: 11.277

4.  Analysis of Pichia pastoris components in recombinant human serum albumin by immunological assays and by HPLC with pulsed amperometric detection.

Authors:  W Ohtani; T Ohda; A Sumi; K Kobayashi; T Ohmura
Journal:  Anal Chem       Date:  1998-01-15       Impact factor: 6.986

5.  Sequence, S-S bridges, and spectra of bovine transcobalamin expressed in Pichia pastoris.

Authors:  S N Fedosov; L Berglund; E Nexo; T E Petersen
Journal:  J Biol Chem       Date:  1999-09-10       Impact factor: 5.157

6.  Expression of human gelatinase B in Pichia pastoris.

Authors:  N Roy; S Padmanabhan; M Smith; L Shi; M Navre; G Das
Journal:  Protein Expr Purif       Date:  1999-07       Impact factor: 1.650

7.  Expression of biologically active human vascular endothelial growth factor in yeast.

Authors:  D Mohanraj; T Olson; S Ramakrishnan
Journal:  Growth Factors       Date:  1995       Impact factor: 2.511

8.  Purification, potency, and efficacy of the botulinum neurotoxin type A binding domain from Pichia pastoris as a recombinant vaccine candidate.

Authors:  M P Byrne; T J Smith; V A Montgomery; L A Smith
Journal:  Infect Immun       Date:  1998-10       Impact factor: 3.441

9.  Production of human tissue factor using the Pichia pastoris expression system.

Authors:  A J Austin; C E Jones; G V Heeke
Journal:  Protein Expr Purif       Date:  1998-06       Impact factor: 1.650

10.  Pichia pastoris as a host system for transformations.

Authors:  J M Cregg; K J Barringer; A Y Hessler; K R Madden
Journal:  Mol Cell Biol       Date:  1985-12       Impact factor: 4.272
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  174 in total

Review 1.  Pharmaceutical strategies utilizing recombinant human serum albumin.

Authors:  Victor Tuan Giam Chuang; Ulrich Kragh-Hansen; Masaki Otagiri
Journal:  Pharm Res       Date:  2002-05       Impact factor: 4.200

2.  Cis-acting elements sufficient for induction of FDH1 expression by formate in the methylotrophic yeast Candida boidinii.

Authors:  T Komeda; H Yurimoto; N Kato; Y Sakai; K Kondo
Journal:  Mol Genet Genomics       Date:  2003-10-08       Impact factor: 3.291

3.  Expression of human augmenter of liver regeneration in pichia pastoris yeast and its bioactivity in vitro.

Authors:  Qi Liu; Hui-Feng Yu; Hang Sun; Hua-Feng Ma
Journal:  World J Gastroenterol       Date:  2004-11-01       Impact factor: 5.742

4.  Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.

Authors:  Anders G Sandström; Ylva Wikmark; Karin Engström; Jonas Nyhlén; Jan-E Bäckvall
Journal:  Proc Natl Acad Sci U S A       Date:  2011-12-16       Impact factor: 11.205

5.  Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures.

Authors:  Petr Hyka; Thomas Züllig; Claudia Ruth; Verena Looser; Christian Meier; Joachim Klein; Karel Melzoch; Hans-Peter Meyer; Anton Glieder; Karin Kovar
Journal:  Appl Environ Microbiol       Date:  2010-05-14       Impact factor: 4.792

6.  Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing.

Authors:  Magali Tanghe; Barbara Danneels; Andrea Camattari; Anton Glieder; Isabel Vandenberghe; Bart Devreese; Ingeborg Stals; Tom Desmet
Journal:  Mol Biotechnol       Date:  2015-12       Impact factor: 2.695

7.  Codon Optimization of the "Bos Taurus Chymosin" Gene for the Production of Recombinant Chymosin in Pichia pastoris.

Authors:  Jose Alberto Espinoza-Molina; Carlos Horacio Acosta-Muñiz; D R Sepulveda; Paul Baruk Zamudio-Flores; Claudio Rios-Velasco
Journal:  Mol Biotechnol       Date:  2016-10       Impact factor: 2.695

8.  Overexpression of an anti-CD3 immunotoxin increases expression and secretion of molecular chaperone BiP/Kar2p by Pichia pastoris.

Authors:  Yuan Yi Liu; Jung Hee Woo; David M Neville
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

9.  Functional expression of trypsin from Streptomyces griseus by Pichia pastoris.

Authors:  Zhenmin Ling; Tengbo Ma; Jianghua Li; Guocheng Du; Zhen Kang; Jian Chen
Journal:  J Ind Microbiol Biotechnol       Date:  2012-07-28       Impact factor: 3.346

10.  Trm1p, a Zn(II)2Cys6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidinii.

Authors:  Yu Sasano; Hiroya Yurimoto; Mikiko Yanaka; Yasuyoshi Sakai
Journal:  Eukaryot Cell       Date:  2008-01-18
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