| Literature DB >> 9862961 |
C Piechaczek1, C Fetzer, A Baiker, J Bode, H J Lipps.
Abstract
We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.Entities:
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Year: 1999 PMID： 9862961 PMCID： PMC148196 DOI： 10.1093/nar/27.2.426
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971