Literature DB >> 9854096

Comparison between PCR and detection of antigen in sera for diagnosis of pulmonary aspergillosis.

S Kawamura1, S Maesaki, T Noda, Y Hirakata, K Tomono, T Tashiro, S Kohno.   

Abstract

We evaluated the usefulness of PCR and antigen detection for the diagnosis of pulmonary aspergillosis. Forty-four serum samples from patients with pulmonary aspergillosis (33 with pulmonary aspergilloma, 4 with allergic bronchopulmonary aspergillosis, 4 with invasive pulmonary aspergillosis, and 3 with aspergillus pyothorax) were used in this study. PCR detection of Aspergillus DNA in serum samples was successful in 39 patients. Galactomannan antigen was detected by sandwich enzyme-linked immunosorbent assay in 25 patients and by latex agglutination test in 13 patients. Detection of Aspergillus DNA in serum samples by nested PCR had the highest sensitivity of the three methods tested for the diagnosis of pulmonary aspergillosis.

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Year:  1999        PMID： 9854096      PMCID： PMC84214     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Avoiding false positives with PCR.

Authors:  S Kwok; R Higuchi
Journal:  Nature       Date:  1989-05-18       Impact factor: 49.962

2.  PCR detection of DNA specific for Aspergillus species in serum of patients with invasive aspergillosis.

Authors:  Y Yamakami; A Hashimoto; I Tokimatsu; M Nasu
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

3.  A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis.

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Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

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Journal:  Ann Intern Med       Date:  1977-04       Impact factor: 25.391

5.  Comparison of an enzyme immunoassay and latex agglutination test for detection of galactomannan in the diagnosis of invasive aspergillosis.

Authors:  A Sulahian; M Tabouret; P Ribaud; J Sarfati; E Gluckman; J P Latgé; F Derouin
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-02       Impact factor: 3.267

6.  Chemical and immunological characterization of the extracellular galactomannan of Aspergillus fumigatus.

Authors:  J P Latgé; H Kobayashi; J P Debeaupuis; M Diaquin; J Sarfati; J M Wieruszeski; E Parra; J P Bouchara; B Fournet
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

7.  Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR.

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Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

8.  Prospective sandwich enzyme-linked immunosorbent assay for serum galactomannan: early predictive value and clinical use in invasive aspergillosis.

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Journal:  Pediatr Infect Dis J       Date:  1996-03       Impact factor: 2.129

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Journal:  Arch Intern Med       Date:  1983-02

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Authors:  C M Tang; D W Holden; A Aufauvre-Brown; J Cohen
Journal:  Am Rev Respir Dis       Date:  1993-11
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  14 in total

1.  Aspergillus antigen testing in bone marrow transplant recipients.

Authors:  E C Williamson; D A Oliver; E M Johnson; A B Foot; D I Marks; D W Warnock
Journal:  J Clin Pathol       Date:  2000-05       Impact factor: 3.411

2.  Comparison of real-time PCR, conventional PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay using bronchoalveolar lavage fluid samples from hematology patients for diagnosis of invasive pulmonary aspergillosis.

Authors:  Maurizio Sanguinetti; Brunella Posteraro; Livio Pagano; Gabriella Pagliari; Luana Fianchi; Luca Mele; Marilena La Sorda; Angelica Franco; Giovanni Fadda
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948

3.  Prospective comparison of the diagnostic potential of real-time PCR, double-sandwich enzyme-linked immunosorbent assay for galactomannan, and a (1-->3)-beta-D-glucan test in weekly screening for invasive aspergillosis in patients with hematological disorders.

Authors:  Masahito Kawazu; Yoshinobu Kanda; Yasuhito Nannya; Katsunori Aoki; Mineo Kurokawa; Shigeru Chiba; Toru Motokura; Hisamaru Hirai; Seishi Ogawa
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

4.  Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis.

Authors:  Laurence Millon; Renaud Piarroux; Eric Deconinck; Claude-Eric Bulabois; Frédéric Grenouillet; Pierre Rohrlich; Jean-Marc Costa; Stéphane Bretagne
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

5.  Real-time PCR coupled with automated DNA extraction and detection of galactomannan antigen in serum by enzyme-linked immunosorbent assay for diagnosis of invasive aspergillosis.

Authors:  Catherine Costa; Jean-Marc Costa; Christophe Desterke; Françoise Botterel; Catherine Cordonnier; Stéphane Bretagne
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

6.  Identification of Aspergillus fumigatus and related species by nested PCR targeting ribosomal DNA internal transcribed spacer regions.

Authors:  J Zhao; F Kong; R Li; X Wang; Z Wan; D Wang
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

7.  Rapid differentiation of Aspergillus species from other medically important opportunistic molds and yeasts by PCR-enzyme immunoassay.

Authors:  Liliana de Aguirre; Steven F Hurst; Jong Soo Choi; Jong Hee Shin; Hans Peter Hinrikson; Christine J Morrison
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

8.  Development and validation of a quantitative real-time PCR assay using fluorescence resonance energy transfer technology for detection of Aspergillus fumigatus in experimental invasive pulmonary aspergillosis.

Authors:  Cathal E O'Sullivan; Miki Kasai; Andrea Francesconi; Vidmantas Petraitis; Ruta Petraitiene; Amy M Kelaher; Alia A Sarafandi; Thomas J Walsh
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

9.  Use of lung resection and voriconazole for successful treatment of invasive pulmonary Aspergillus ustus infection.

Authors:  A Azzola; J R Passweg; J M Habicht; L Bubendorf; M Tamm; A Gratwohl; G Eich
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

Review 10.  Current status of nonculture methods for diagnosis of invasive fungal infections.

Authors:  Siew Fah Yeo; Brian Wong
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132
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