Literature DB >> 9835595

Utility of green fluorescent nucleic acid dyes and aluminum oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria.

M G Weinbauer1, C Beckmann, M G Höfle.   

Abstract

High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4',6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane filters. Due to the bright staining of cells, we recommend SYBR Green II as dye.

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Year:  1998        PMID： 9835595      PMCID： PMC90955     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Comparison of a new inorganic membrane filter (Anopore) with a track-etched polycarbonate membrane filter (Nuclepore) for direct counting of bacteria.

Authors:  S E Jones; S A Ditner; C Freeman; C J Whitaker; M A Lock
Journal:  Appl Environ Microbiol       Date:  1989-02       Impact factor: 4.792

2.  Solutions to problems in enumerating sediment bacteria by direct counts.

Authors:  M Schallenberg; J Kalff; J B Rasmussen
Journal:  Appl Environ Microbiol       Date:  1989-05       Impact factor: 4.792

3.  Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.

Authors:  A B Dahle; M Laake
Journal:  Appl Environ Microbiol       Date:  1982-01       Impact factor: 4.792

4.  Fully automatic determination of soil bacterium numbers, cell volumes, and frequencies of dividing cells by confocal laser scanning microscopy and image analysis.

Authors:  J Bloem; M Veninga; J Shepherd
Journal:  Appl Environ Microbiol       Date:  1995-03       Impact factor: 4.792

5.  Enumeration and Cell Cycle Analysis of Natural Populations of Marine Picoplankton by Flow Cytometry Using the Nucleic Acid Stain SYBR Green I.

Authors:  D Marie; F Partensky; S Jacquet; D Vaulot
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

6.  Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.

Authors:  P Lebaron; N Parthuisot; P Catala
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

Review 7.  Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present.

Authors:  R L Kepner; J R Pratt
Journal:  Microbiol Rev       Date:  1994-12

8.  Total counts of marine bacteria include a large fraction of non-nucleoid-containing bacteria (ghosts).

Authors:  U L Zweifel; A Hagstrom
Journal:  Appl Environ Microbiol       Date:  1995-06       Impact factor: 4.792

9.  Degradation of chloro- and methyl-substituted benzoic acids by a genetically modified microorganism.

Authors:  R Müller; W D Deckwer; V Hecht
Journal:  Biotechnol Bioeng       Date:  1996-09-05       Impact factor: 4.530

10.  Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes.

Authors:  D Marie; D Vaulot; F Partensky
Journal:  Appl Environ Microbiol       Date:  1996-05       Impact factor: 4.792
  10 in total
  35 in total

1.  Simultaneous direct counting of total and specific microbial cells in seawater, using a deep-sea microbe as target.

Authors:  A Maruyama; M Sunamura
Journal:  Appl Environ Microbiol       Date:  2000-05       Impact factor: 4.792

2.  Rapid virus production and removal as measured with fluorescently labeled viruses as tracers.

Authors:  R T Noble; J A Fuhrman
Journal:  Appl Environ Microbiol       Date:  2000-09       Impact factor: 4.792

3.  Large fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols for determination and ecological significance.

Authors:  G M Luna; E Manini; R Danovaro
Journal:  Appl Environ Microbiol       Date:  2002-07       Impact factor: 4.792

4.  A model of genetic search for beneficial mutations: estimating the constructive capacities of mutagenesis.

Authors:  Grigory G Ananko
Journal:  J Mol Evol       Date:  2012-01-03       Impact factor: 2.395

5.  High-resolution in situ genotyping of Legionella pneumophila populations in drinking water by multiple-locus variable-number tandem-repeat analysis using environmental DNA.

Authors:  Leila Kahlisch; Karsten Henne; Josefin Draheim; Ingrid Brettar; Manfred G Höfle
Journal:  Appl Environ Microbiol       Date:  2010-07-23       Impact factor: 4.792

6.  Sensitive determination of microbial growth by nucleic acid staining in aqueous suspension.

Authors:  Willm Martens-Habbena; Henrik Sass
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

7.  Dissemination and survival of non-indigenous bacterial genomes in pristine Antarctic environments.

Authors:  Lemese Ah Tow; Don A Cowan
Journal:  Extremophiles       Date:  2005-06-10       Impact factor: 2.395

8.  Infection paradox: high abundance but low impact of freshwater benthic viruses.

Authors:  Manuela Filippini; Nanna Buesing; Yvan Bettarel; Télesphore Sime-Ngando; Mark O Gessner
Journal:  Appl Environ Microbiol       Date:  2006-07       Impact factor: 4.792

9.  Phylogenetic and physiological diversity of bacteria isolated from Puruogangri ice core.

Authors:  X F Zhang; T D Yao; L D Tian; S J Xu; L Z An
Journal:  Microb Ecol       Date:  2007-09-18       Impact factor: 4.552

10.  Prokaryotic metabolic activity and community structure in Antarctic continental shelf sediments.

Authors:  J P Bowman; S A McCammon; J A E Gibson; L Robertson; P D Nichols
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792
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