Literature DB >> 9758846

Purification and characterization of an NAD-malic enzyme from Bradyrhizobium japonicum A1017.

F Chen1, Y Okabe, K Osano, S Tajima.   

Abstract

An NAD-malic enzyme was purified to homogeneity from Bradyrhizobium japonicum A1017, and its molecular characteristics were surveyed. The enzyme exhibited native and subunit molecular masses of 388 and 85 kDa, respectively, suggesting that it exists as a homotetramer, and was activated by metabolic intermediates in glycolysis. The role of the enzyme in bacteroids' carbon metabolism is discussed.

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Year:  1998        PMID: 9758846      PMCID: PMC106605     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  14 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Purification and characterization of the NADP-malic enzyme from Bradyrhizobium japonicum A1017.

Authors:  F Chen; Y Okabe; K Osano; S Tajima
Journal:  Biosci Biotechnol Biochem       Date:  1997-02       Impact factor: 2.043

3.  Physiological functions of NAD- and NADP-linked malic enzymes in Escherichia coli.

Authors:  T Murai; M Tokushige; J Nagai; H Katsuki
Journal:  Biochem Biophys Res Commun       Date:  1971-05-21       Impact factor: 3.575

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Studies on regulatory functions of malic enzymes. II. Purification and molecular properties of nicotinamide adenine dinucleotide-linked malic enzyme from Eschericha coli.

Authors:  M Yamaguchi; M Tokushige; H Katsuki
Journal:  J Biochem       Date:  1973-01       Impact factor: 3.387

6.  Plant mitochondrial NAD+-dependent malic enzyme. cDNA cloning, deduced primary structure of the 59- and 62-kDa subunits, import, gene complexity and expression analysis.

Authors:  B M Winning; J Bourguignon; C J Leaver
Journal:  J Biol Chem       Date:  1994-02-18       Impact factor: 5.157

7.  Mitochondrial malic enzymes. Purification and properties of the NAD(P)-dependent malic enzyme from canine small intestinal mucosa.

Authors:  W O Nagel; L A Sauer
Journal:  J Biol Chem       Date:  1982-10-25       Impact factor: 5.157

8.  NAD(+)-dependent malic enzyme of Rhizobium meliloti is required for symbiotic nitrogen fixation.

Authors:  B T Driscoll; T M Finan
Journal:  Mol Microbiol       Date:  1993-03       Impact factor: 3.501

9.  Structure and properties of malic enzyme from Bacillus stearothermophilus.

Authors:  K Kobayashi; S Doi; S Negoro; I Urabe; H Okada
Journal:  J Biol Chem       Date:  1989-02-25       Impact factor: 5.157

10.  Mechanism of activation of the NAD-malic enzyme from Ascaris suum by fumarate.

Authors:  C J Lai; B G Harris; P F Cook
Journal:  Arch Biochem Biophys       Date:  1992-12       Impact factor: 4.013

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4.  Malic enzyme cofactor and domain requirements for symbiotic N2 fixation by Sinorhizobium meliloti.

Authors:  Michael J Mitsch; Alison Cowie; Turlough M Finan
Journal:  J Bacteriol       Date:  2006-10-27       Impact factor: 3.490

5.  Role of the malic enzyme in metabolism of the halotolerant methanotroph Methylotuvimicrobium alcaliphilum 20Z.

Authors:  Olga N Rozova; Ildar I Mustakhimov; Sergei Y But; Aleksandr S Reshetnikov; Valentina N Khmelenina
Journal:  PLoS One       Date:  2019-11-18       Impact factor: 3.240

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