OBJECTIVE: Assessing the stability of cefazolin sodium in preservative-free and preservative-containing eye drops. METHOD: Extemporaneous formulations of eye drops were prepared from a commercially-available parenteral product of cefazolin sodium: eye drops 'A' contained 50mg/ml of cefazolin sodium in 0.45% w/v sodium chloride solution, and eye drops 'B' contained 50 mg/ml, 0.005% w/v thiomersal and 1% w/v glycerol in water-for-injection. Cefazolin sodium concentrations in these eye drops were monitored by a stability-indicating HPLC assay method. Measurements of pH and osmolality, as well as tests for microbial contamination, were conducted. RESULTS: The eye drops stored at 4 degrees C were stable for 42 days with minimal changes in pH and osmolality, but eye drops stored at room temperature were only stable for a few days with greater increments in pH and osmolality. None of the samples cultured had bacterial or fungal growth after 7 days of incubation. CONCLUSION: Extemporaneously prepared formulations of cefazolin are unstable at room temperature and should be stored in a refrigerator.
OBJECTIVE: Assessing the stability of cefazolin sodium in preservative-free and preservative-containing eye drops. METHOD: Extemporaneous formulations of eye drops were prepared from a commercially-available parenteral product of cefazolin sodium: eye drops 'A' contained 50mg/ml of cefazolin sodium in 0.45% w/v sodium chloride solution, and eye drops 'B' contained 50 mg/ml, 0.005% w/v thiomersal and 1% w/v glycerol in water-for-injection. Cefazolin sodium concentrations in these eye drops were monitored by a stability-indicating HPLC assay method. Measurements of pH and osmolality, as well as tests for microbial contamination, were conducted. RESULTS: The eye drops stored at 4 degrees C were stable for 42 days with minimal changes in pH and osmolality, but eye drops stored at room temperature were only stable for a few days with greater increments in pH and osmolality. None of the samples cultured had bacterial or fungal growth after 7 days of incubation. CONCLUSION: Extemporaneously prepared formulations of cefazolin are unstable at room temperature and should be stored in a refrigerator.
Authors: Daoud Al-Badriyeh; Lok Leung; Trent Roydhouse; Robert Fullinfaw; Mark Daniell; Geoffrey E Davies; Kay Stewart; David C M Kong Journal: Antimicrob Agents Chemother Date: 2009-05-11 Impact factor: 5.191
Authors: Chin Fen Neoh; Jovan Jacob; Lok Leung; Jian Li; Angela Stathopoulos; Kay Stewart; David C M Kong Journal: Antimicrob Agents Chemother Date: 2012-04-02 Impact factor: 5.191
Authors: Chin Fen Neoh; Lok Leung; Anant Misra; Rasik B Vajpayee; Geoffrey E Davies; Robert O Fullinfaw; Kay Stewart; David C M Kong Journal: Antimicrob Agents Chemother Date: 2011-01-24 Impact factor: 5.191