M Bauchinger1, E Schmid. 1. GSF, National Research Center for Environment and Health, Institute of Radiobiology, Neuherberg, Germany.
Abstract
PURPOSE: To investigate the LET dependence of various yield ratios of intra- and interchromosomal aberrations in order to quantify proximity effects for illegitimate rejoining of chromosome breaks. MATERIALS AND METHODS: Calculations are based on 13 human lymphocyte data sets obtained from in vitro irradiation experiments with various radiation qualities covering an LET range of 0.5 to 150keV/microm. A total of 93000 first-division metaphases was analysed for all categories of chromosome-type aberrations. RESULTS: No dose dependence was found for the various yield ratios of chromosomal aberrations. No LET dependence became apparent for the yield ratio of inter- to intrachromosomal exchange-type aberrations (dicentrics/centric rings = F value). However, a clear LET dependence was found for the yield ratio of intra-arm intrachanges to inter-arm intrachanges (interstitial deletions/centric rings = G ratio) and for the yield ratio of intra-arm intrachanges to interchromosomal exchanges (interstitial deletions/dicentrics = H ratio). CONCLUSION: Measurements of intrachanges are informative for interpretation and quantification of proximity effects in the formation of radiation-induced chromosome aberrations. In particular, yield ratios for intra-arm intrachanges are likely to have a high potential as an indicator of high-LET radiations.
PURPOSE: To investigate the LET dependence of various yield ratios of intra- and interchromosomal aberrations in order to quantify proximity effects for illegitimate rejoining of chromosome breaks. MATERIALS AND METHODS: Calculations are based on 13 human lymphocyte data sets obtained from in vitro irradiation experiments with various radiation qualities covering an LET range of 0.5 to 150keV/microm. A total of 93000 first-division metaphases was analysed for all categories of chromosome-type aberrations. RESULTS: No dose dependence was found for the various yield ratios of chromosomal aberrations. No LET dependence became apparent for the yield ratio of inter- to intrachromosomal exchange-type aberrations (dicentrics/centric rings = F value). However, a clear LET dependence was found for the yield ratio of intra-arm intrachanges to inter-arm intrachanges (interstitial deletions/centric rings = G ratio) and for the yield ratio of intra-arm intrachanges to interchromosomal exchanges (interstitial deletions/dicentrics = H ratio). CONCLUSION: Measurements of intrachanges are informative for interpretation and quantification of proximity effects in the formation of radiation-induced chromosome aberrations. In particular, yield ratios for intra-arm intrachanges are likely to have a high potential as an indicator of high-LET radiations.
Authors: M Prakash Hande; Tamara V Azizova; Charles R Geard; Ludmilla E Burak; Catherine R Mitchell; Valentin F Khokhryakov; Evgeny K Vasilenko; David J Brenner Journal: Am J Hum Genet Date: 2003-04-04 Impact factor: 11.025
Authors: E Schmid; H-J Selbach; M Voth; J Pinkert; F J Gildehaus; R Klett; M Haney Journal: Radiat Environ Biophys Date: 2006-05-30 Impact factor: 1.925
Authors: Qi Wang; Younghyun Lee; Monica Pujol-Canadell; Jay R Perrier; Lubomir Smilenov; Andrew Harken; Guy Garty; David J Brenner; Brian Ponnaiya; Helen C Turner Journal: Cytogenet Genome Res Date: 2021-09-06 Impact factor: 1.941
Authors: Terri L Ryan; Antonio G Pantelias; Georgia I Terzoudi; Gabriel E Pantelias; Adayabalam S Balajee Journal: PLoS One Date: 2019-05-06 Impact factor: 3.240