Literature DB >> 9650980

Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR.

B Jaulhac1, M Reyrolle, Y K Sodahlon, S Jarraud, M Kubina, H Monteil, Y Piémont, J Etienne.   

Abstract

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate.

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Year:  1998        PMID： 9650980      PMCID： PMC104998     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  A rapid method for preparing DNA from blood, suited for PCR screening of transgenes in mice.

Authors:  G Winberg
Journal:  PCR Methods Appl       Date:  1991-08

2.  Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

Authors:  P S Walsh; D A Metzger; R Higuchi
Journal:  Biotechniques       Date:  1991-04       Impact factor: 1.993

3.  A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.

Authors:  X de Lamballerie; C Zandotti; C Vignoli; C Bollet; P de Micco
Journal:  Res Microbiol       Date:  1992-10       Impact factor: 3.992

4.  Detection of Legionella spp. in bronchoalveolar lavage fluids by DNA amplification.

Authors:  B Jaulhac; M Nowicki; N Bornstein; O Meunier; G Prevost; Y Piemont; J Fleurette; H Monteil
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

5.  Use of polymerase chain reaction in an epidemiologic investigation of Pontiac fever.

Authors:  L A Miller; J L Beebe; J C Butler; W Martin; R Benson; R E Hoffman; B S Fields
Journal:  J Infect Dis       Date:  1993-09       Impact factor: 5.226

Review 6.  Legionnaires' disease.

Authors:  P H Edelstein
Journal:  Clin Infect Dis       Date:  1993-06       Impact factor: 9.079

7.  Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit.

Authors:  H H Kessler; F F Reinthaler; A Pschaid; K Pierer; B Kleinhappl; E Eber; E Marth
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

8.  Evaluation of commercial amplification kit for detection of Legionella pneumophila in clinical specimens.

Authors:  P Matsiota-Bernard; E Pitsouni; N Legakis; C Nauciel
Journal:  J Clin Microbiol       Date:  1994-06       Impact factor: 5.948

9.  Construction of a DNA amplification assay for detection of Legionella species in clinical samples.

Authors:  G Lisby; R Dessau
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-03       Impact factor: 3.267

10.  Comparison of sample preparation methods for detection of Chlamydia pneumoniae in bronchoalveolar lavage fluid by PCR.

Authors:  M Maass; K Dalhoff
Journal:  J Clin Microbiol       Date:  1994-10       Impact factor: 5.948
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  4 in total

1.  Detection of Herpes simplex virus DNA by real-time PCR.

Authors:  H H Kessler; G Mühlbauer; B Rinner; E Stelzl; A Berger; H W Dörr; B Santner; E Marth; H Rabenau
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

2.  Nonculture Laboratory Methods for the Diagnosis of Infectious Endocarditis.

Authors: 
Journal:  Curr Infect Dis Rep       Date:  1999-06       Impact factor: 3.725

3.  Legionella.

Authors: 
Journal:  Curr Infect Dis Rep       Date:  1999-04       Impact factor: 3.725

4.  Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis.

Authors:  M J Loeffelholz; C J Thompson; K S Long; M J Gilchrist
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948
  4 in total

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