Literature DB >> 1298031

A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.

X de Lamballerie1, C Zandotti, C Vignoli, C Bollet, P de Micco.   

Abstract

"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.

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Year:  1992        PMID： 1298031     DOI： 10.1016/0923-2508(92)90107-y

Source DB:  PubMed          Journal:  Res Microbiol        ISSN： 0923-2508            Impact factor:   3.992


  88 in total

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4.  Novel multipurpose methodology for detection of mycobacteria in pulmonary and extrapulmonary specimens by smear microscopy, culture, and PCR.

Authors:  Soumitesh Chakravorty; Jaya Sivaswami Tyagi
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

5.  Detection of acid-fast bacilli in postlysis debris of clinical specimens improves the reliability of PCR.

Authors:  Sagarika Haldar; Shyamasree De Majumdar; Soumitesh Chakravorty; Jaya Sivaswami Tyagi; Manpreet Bhalla; M K Sen
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6.  Biochemical bases of type IV chromatic adaptation in marine Synechococcus spp.

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7.  Inactivation of cultured Mycobacterium tuberculosis organisms prior to DNA extraction.

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Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948

8.  Multiplex PCR assay for typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.

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Journal:  J Vis Exp       Date:  2013-09-05       Impact factor: 1.355

9.  Detection of JC virus by polymerase chain reaction and colorimetric DNA hybridization assay.

Authors:  C Vignoli; X De Lamballerie; C Zandotti; C Tamalet; P De Micco
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-12       Impact factor: 3.267

10.  Efficient discrimination of Mycobacterium tuberculosis strains by 16S-23S spacer region-based random amplified polymorphic DNA analysis.

Authors:  Y Abed; A Davin-Regli; C Bollet; P De Micco
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948
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