Literature DB >> 9647851

Effectiveness of SYTOX Green stain for bacterial viability assessment.

P Lebaron1, P Catala, N Parthuisot.   

Abstract

The effectiveness of SYTOX Green nucleic acid stain for measuring bacterial viability was tested on starved populations of Escherichia coli and Salmonella typhimurium. This stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. Its application to natural samples should be considered with caution.

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Year:  1998        PMID: 9647851      PMCID: PMC106447     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  15 in total

Review 1.  Physiological assessment of bacteria using fluorochromes.

Authors:  G A McFeters; F P Yu; B H Pyle; P S Stewart
Journal:  J Microbiol Methods       Date:  1995-01       Impact factor: 2.363

2.  Development of a robust flow cytometric assay for determining numbers of viable bacteria.

Authors:  R I Jepras; J Carter; S C Pearson; F E Paul; M J Wilkinson
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

3.  Enumeration and Cell Cycle Analysis of Natural Populations of Marine Picoplankton by Flow Cytometry Using the Nucleic Acid Stain SYBR Green I.

Authors:  D Marie; F Partensky; S Jacquet; D Vaulot
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

4.  Direct measurements of natural planktonic bacterial community viability by flow cytometry.

Authors:  J Porter; J Diaper; C Edwards; R Pickup
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

5.  Role of ribosome degradation in the death of starved Escherichia coli cells.

Authors:  B D Davis; S M Luger; P C Tai
Journal:  J Bacteriol       Date:  1986-05       Impact factor: 3.490

6.  In situ analysis of nucleic acids in cold-induced nonculturable Vibrio vulnificus.

Authors:  D Weichart; D McDougald; D Jacobs; S Kjelleberg
Journal:  Appl Environ Microbiol       Date:  1997-07       Impact factor: 4.792

7.  Flow cytometry for rapid assessment of viability after exposure to a quaternary ammonium compound.

Authors:  S Langsrud; G Sundheim
Journal:  J Appl Bacteriol       Date:  1996-10

8.  Total counts of marine bacteria include a large fraction of non-nucleoid-containing bacteria (ghosts).

Authors:  U L Zweifel; A Hagstrom
Journal:  Appl Environ Microbiol       Date:  1995-06       Impact factor: 4.792

9.  Determination of Active Marine Bacterioplankton: a Comparison of Universal 16S rRNA Probes, Autoradiography, and Nucleoid Staining.

Authors:  M Karner; J A Fuhrman
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792

10.  Flow cytometric analysis of the cellular DNA content of Salmonella typhimurium and Alteromonas haloplanktis during starvation and recovery in seawater.

Authors:  P Lebaron; F Joux
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792

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  38 in total

1.  Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments.

Authors:  D Yokomaku; N Yamaguchi; M Nasu
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Effects of D-Lysine Substitutions on the Activity and Selectivity of Antimicrobial Peptide CM15.

Authors:  Heather M Kaminski; Jimmy B Feix
Journal:  Polymers (Basel)       Date:  2011-12-06       Impact factor: 4.329

3.  Reliable detection of dead microbial cells by using fluorescent hydrazides.

Authors:  Claude Saint-Ruf; Corinne Cordier; Jérome Mégret; Ivan Matic
Journal:  Appl Environ Microbiol       Date:  2009-12-28       Impact factor: 4.792

4.  Flow cytometric monitoring of antibiotic-induced injury in Escherichia coli using cell-impermeant fluorescent probes.

Authors:  F C Mortimer; D J Mason; V A Gant
Journal:  Antimicrob Agents Chemother       Date:  2000-03       Impact factor: 5.191

5.  Rapid assessment of the physiological status of the polychlorinated biphenyl degrader Comamonas testosteroni TK102 by flow cytometry.

Authors:  Yoshinori Hiraoka; Kazuhide Kimbara
Journal:  Appl Environ Microbiol       Date:  2002-04       Impact factor: 4.792

6.  Inactivation of Bacteria by γ-Irradiation to Investigate the Interaction with Antimicrobial Peptides.

Authors:  Wilmar Correa; Julius Brandenburg; Jochen Behrends; Lena Heinbockel; Norbert Reiling; Laura Paulowski; Dominik Schwudke; Kerstin Stephan; Guillermo Martinez-de-Tejada; Klaus Brandenburg; Thomas Gutsmann
Journal:  Biophys J       Date:  2019-10-18       Impact factor: 4.033

7.  Flow cytometric assessment of viability of lactic acid bacteria.

Authors:  C J Bunthof; K Bloemen; P Breeuwer; F M Rombouts; T Abee
Journal:  Appl Environ Microbiol       Date:  2001-05       Impact factor: 4.792

8.  Evaluation of antibacterial activity against Salmonella Enteritidis.

Authors:  Gaëlle Legendre; Fabienne Faÿ; Isabelle Linossier; Karine Vallée-Réhel
Journal:  J Microbiol       Date:  2011-06-30       Impact factor: 3.422

9.  Decoding the molecular properties of mycobacteriophage D29 Holin provides insights into Holin engineering.

Authors:  Varun Rakeshbhai Bavda; Aditi Yadav; Vikas Jain
Journal:  J Virol       Date:  2021-02-24       Impact factor: 5.103

10.  Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in Candida albicans.

Authors:  Ligia Maria Giroldo; Monalisa Poliana Felipe; Marco Antonio de Oliveira; Egberto Munin; Leandro Procópio Alves; Maricilia Silva Costa
Journal:  Lasers Med Sci       Date:  2007-12-22       Impact factor: 3.161

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