Literature DB >> 16535084

Direct measurements of natural planktonic bacterial community viability by flow cytometry.

J Porter, J Diaper, C Edwards, R Pickup.   

Abstract

A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.

Entities:  

Year:  1995        PMID： 16535084      PMCID： PMC1388502          DOI： 10.1128/aem.61.7.2783-2786.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  Measurement of intracellular pH.

Authors:  E A Musgrove; D W Hedley
Journal:  Methods Cell Biol       Date:  1990       Impact factor: 1.441

2.  Isolation of Typical Marine Bacteria by Dilution Culture: Growth, Maintenance, and Characteristics of Isolates under Laboratory Conditions.

Authors:  F Schut; E J de Vries; J C Gottschal; B R Robertson; W Harder; R A Prins; D K Button
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792

3.  Response of marine bacterioplankton to differential filtration and confinement.

Authors:  R L Ferguson; E N Buckley; A V Palumbo
Journal:  Appl Environ Microbiol       Date:  1984-01       Impact factor: 4.792

4.  An investigation of errors in direct counts of aquatic bacteria by epifluorescence microscopy, with reference to a new method for dyeing membrane filters.

Authors:  J G Jones; B M Simon
Journal:  J Appl Bacteriol       Date:  1975-12

5.  Use of a fluorescent redox probe for direct visualization of actively respiring bacteria.

Authors:  G G Rodriguez; D Phipps; K Ishiguro; H F Ridgway
Journal:  Appl Environ Microbiol       Date:  1992-06       Impact factor: 4.792

6.  Survival of nonculturable Aeromonas salmonicida in lake water.

Authors:  J A Morgan; G Rhodes; R W Pickup
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

7.  Lymphocyte membrane potential and Ca2+-sensitive potassium channels described by oxonol dye fluorescence measurements.

Authors:  H A Wilson; T M Chused
Journal:  J Cell Physiol       Date:  1985-10       Impact factor: 6.384

8.  Rapid assessment of bacterial viability by flow cytometry.

Authors:  J P Diaper; K Tither; C Edwards
Journal:  Appl Microbiol Biotechnol       Date:  1992-11       Impact factor: 4.813

9.  Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting.

Authors:  J Porter; C Edwards; J A Morgan; R W Pickup
Journal:  Appl Environ Microbiol       Date:  1993-10       Impact factor: 4.792

10.  Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae.

Authors:  J Dorsey; C M Yentsch; S Mayo; C McKenna
Journal:  Cytometry       Date:  1989-09
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  11 in total

1.  Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments.

Authors:  D Yokomaku; N Yamaguchi; M Nasu
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescence techniques.

Authors:  Liisa Nohynek; Eija Saski; Auli Haikara; Laura Raaska
Journal:  J Ind Microbiol Biotechnol       Date:  2003-04-26       Impact factor: 3.346

3.  Bacterial abundance, activity, and viability in the eutrophic River Warnow, northeast Germany.

Authors:  H M Freese; U Karsten; R Schumann
Journal:  Microb Ecol       Date:  2006-01-11       Impact factor: 4.552

4.  Effectiveness of SYTOX Green stain for bacterial viability assessment.

Authors:  P Lebaron; P Catala; N Parthuisot
Journal:  Appl Environ Microbiol       Date:  1998-07       Impact factor: 4.792

5.  Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain.

Authors:  B L Roth; M Poot; S T Yue; P J Millard
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

Review 6.  Quantification of the presence and activity of specific microorganisms in nature.

Authors:  J K Jansson; J I Prosser
Journal:  Mol Biotechnol       Date:  1997-04       Impact factor: 2.695

7.  Changes in Cellular States of the Marine Bacterium Deleya aquamarina under Starvation Conditions.

Authors:  F Joux; P Lebaron; M Troussellier
Journal:  Appl Environ Microbiol       Date:  1997-07       Impact factor: 4.792

8.  Effect of trophic status on the culturability and activity of bacteria from a range of lakes in the English Lake District.

Authors:  Jonathan Porter; Samantha A Morris; Roger W Pickup
Journal:  Appl Environ Microbiol       Date:  2004-04       Impact factor: 4.792

9.  Combined Systems Approaches Reveal a Multistage Mode of Action of a Marine Antimicrobial Peptide against Pathogenic Escherichia coli and Its Protective Effect against Bacterial Peritonitis and Endotoxemia.

Authors:  Xiumin Wang; Da Teng; Ruoyu Mao; Na Yang; Ya Hao; Jianhua Wang
Journal:  Antimicrob Agents Chemother       Date:  2016-12-27       Impact factor: 5.191

10.  An improved cell separation technique for marine subsurface sediments: applications for high-throughput analysis using flow cytometry and cell sorting.

Authors:  Yuki Morono; Takeshi Terada; Jens Kallmeyer; Fumio Inagaki
Journal:  Environ Microbiol       Date:  2013-06-03       Impact factor: 5.491
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