Literature DB >> 9608744

Identification of clinically important species of Enterococcus within 1 day with randomly amplified polymorphic DNA (RAPD).

M Quednau1, S Ahrné, A C Petersson, G Molin.   

Abstract

The use of randomly amplified polymorphic DNA (RAPD) for rapid, reliable, and easily interpreted identification of enterococci was evaluated. Nineteen type strains of Enterococcus, 12 reference strains, and 114 clinical isolates of Enterococcus were analyzed. Discrimination was obtained between most type strains, the exceptions being Ent. casseliflavus and Ent. flavescens, which had relatively similar RAPD-profiles. Ent. faecalis and Ent. faecium were readily separated, and Ent. gallinarum and Ent. durans could also be identified. Extracts to be used in the polymerase chain reaction (PCR) were prepared directly from agar plate colonies, which made it possible to complete the identification procedure in one day. RAPD was proved to be a fast and reliable method for identification of most Enterococcus spp. of clinical significance.

Mesh：

Year:  1998        PMID： 9608744     DOI： 10.1007/s002849900318

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  15 in total

1.  Development of a PCR assay for rapid detection of enterococci.

Authors:  D Ke; F J Picard; F Martineau; C Ménard; P H Roy; M Ouellette; M G Bergeron
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

2.  Genomic relationships between Enterococcus faecium strains from different sources and with different antibiotic resistance profiles evaluated by restriction endonuclease analysis of total chromosomal DNA using EcoRI and PvuII.

Authors:  M Quednau; S Ahrné; G Molin
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

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Authors:  Peter Mangell; Henrik Thorlacius; Ingvar Syk; Siv Ahrné; Göran Molin; Crister Olsson; Bengt Jeppsson
Journal:  Dig Dis Sci       Date:  2012-03-21       Impact factor: 3.199

5.  Role of house flies in the ecology of Enterococcus faecalis from wastewater treatment facilities.

Authors:  C W Doud; H M Scott; L Zurek
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Journal:  BMC Womens Health       Date:  2011-05-25       Impact factor: 2.809

7.  Application of tRNA intergenic spacer PCR for identification of Enterococcus species.

Authors:  M Baele; P Baele; M Vaneechoutte; V Storms; P Butaye; L A Devriese; G Verschraegen; M Gillis; F Haesebrouck
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

8.  Vaginal lactobacillus flora of healthy Swedish women.

Authors:  Alejandra Vásquez; Tell Jakobsson; Siv Ahrné; Urban Forsum; Göran Molin
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

9.  Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium.

Authors:  Nadia Osman; Diya Adawi; Siv Ahrne; Bengt Jeppsson; Göran Molin
Journal:  Dig Dis Sci       Date:  2004-02       Impact factor: 3.199

10.  Probiotics and blueberry attenuate the severity of dextran sulfate sodium (DSS)-induced colitis.

Authors:  Nadia Osman; Diya Adawi; Siv Ahrné; Bengt Jeppsson; Göran Molin
Journal:  Dig Dis Sci       Date:  2008-02-15       Impact factor: 3.199
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