Literature DB >> 11060090

Application of tRNA intergenic spacer PCR for identification of Enterococcus species.

M Baele1, P Baele, M Vaneechoutte, V Storms, P Butaye, L A Devriese, G Verschraegen, M Gillis, F Haesebrouck.   

Abstract

tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highly similar though distinctive patterns.

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Year:  2000        PMID： 11060090      PMCID： PMC87563     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  29 in total

1.  Rapid and accurate identification of Staphylococcus species by tRNA intergenic spacer length polymorphism analysis.

Authors:  N Maes; Y De Gheldre; R De Ryck; M Vaneechoutte; H Meugnier; J Etienne; M J Struelens
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Recent approaches on the taxonomy of the enterococci and some related microorganisms.

Authors:  L M Teixeira; M G Carvalho; V L Merquior; A G Steigerwalt; M G Teixeira; D J Brenner; R R Facklam
Journal:  Adv Exp Med Biol       Date:  1997       Impact factor: 2.622

3.  Identification of clinically important species of Enterococcus within 1 day with randomly amplified polymorphic DNA (RAPD).

Authors:  M Quednau; S Ahrné; A C Petersson; G Molin
Journal:  Curr Microbiol       Date:  1998-06       Impact factor: 2.188

4.  Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates.

Authors:  M Vaneechoutte; P Boerlin; H V Tichy; E Bannerman; B Jäger; J Bille
Journal:  Int J Syst Bacteriol       Date:  1998-01

5.  Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human medicine.

Authors:  P Descheemaeker; C Lammens; B Pot; P Vandamme; H Goossens
Journal:  Int J Syst Bacteriol       Date:  1997-04

6.  Acidification of methyl-alpha-D-glucopyranoside: a useful test to differentiate Enterococcus casseliflavus and Enterococcus gallinarum from Enterococcus faecium species group and from Enterococcus faecalis.

Authors:  L A Devriese; B Pot; K Kersters; S Lauwers; F Haesebrouck
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

7.  Proficiency of clinical laboratories in Spain in detecting vancomycin-resistant Enterococcus spp. The Spanish VRE Study Group.

Authors:  J Alonso-Echanove; B Robles; W R Jarvis
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

8.  Identification of clinically relevant viridans streptococci by analysis of transfer DNA intergenic spacer length polymorphism.

Authors:  Y De Gheldre; P Vandamme; H Goossens; M J Struelens
Journal:  Int J Syst Bacteriol       Date:  1999-10

9.  Clinical and epidemiological significance of enterococci intrinsically resistant to vancomycin (possessing the vanC genotype)

Authors:  B Toye; J Shymanski; M Bobrowska; W Woods; K Ramotar
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

10.  Species identification of enterococci via intergenic ribosomal PCR.

Authors:  G J Tyrrell; R N Bethune; B Willey; D E Low
Journal:  J Clin Microbiol       Date:  1997-05       Impact factor: 5.948
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  42 in total

1.  One case each of recurrent meningitis and hemoperitoneum infection with Ralstonia mannitolilytica.

Authors:  M Vaneechoutte; T De Baere; G Wauters; S Steyaert; G Claeys; D Vogelaers; G Verschraegen
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

2.  Use of a genus- and species-specific multiplex PCR for identification of enterococci.

Authors:  Charlene R Jackson; Paula J Fedorka-Cray; John B Barrett
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

3.  Bacterial colonization on fecal pellets of harpacticoid copepods and on their diatom food.

Authors:  Marleen De Troch; Clio Cnudde; Anne Willems; Tom Moens; Ann Vanreusel
Journal:  Microb Ecol       Date:  2010-05-04       Impact factor: 4.552

4.  Naturally transformable Acinetobacter sp. strain ADP1 belongs to the newly described species Acinetobacter baylyi.

Authors:  Mario Vaneechoutte; David M Young; L Nicholas Ornston; Thierry De Baere; Alexandr Nemec; Tanny Van Der Reijden; Emma Carr; Ingela Tjernberg; Lenie Dijkshoorn
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

5.  Evaluation of tRNA gene PCR for identification of mollicutes.

Authors:  Tim Stakenborg; Jo Vicca; Rita Verhelst; Patrick Butaye; Dominiek Maes; Anne Naessens; Geert Claeys; Catharine De Ganck; Freddy Haesebrouck; Mario Vaneechoutte
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

6.  Array-based identification of species of the genera Abiotrophia, Enterococcus, Granulicatella, and Streptococcus.

Authors:  Sheng Kai Tung; Lee Jene Teng; Mario Vaneechoutte; Hung Mo Chen; Tsung Chain Chang
Journal:  J Clin Microbiol       Date:  2006-10-25       Impact factor: 5.948

7.  Use of the manganese-dependent superoxide dismutase gene sodA for rapid identification of recently described enterococcal species.

Authors:  Petra Frolkova; Anuradha Ghosh; Pavel Svec; Ludek Zurek; Ivan Literak
Journal:  Folia Microbiol (Praha)       Date:  2012-05-09       Impact factor: 2.099

8.  Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora.

Authors:  Nabil Abdullah El Aila; Inge Tency; Geert Claeys; Hans Verstraelen; Bart Saerens; Guido Lopes Dos Santos Santiago; Ellen De Backer; Piet Cools; Marleen Temmerman; Rita Verhelst; Mario Vaneechoutte
Journal:  BMC Infect Dis       Date:  2009-10-14       Impact factor: 3.090

9.  Genotyping of Streptococcus agalactiae (group B streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancy.

Authors:  Nabil Abdullah El Aila; Inge Tency; Geert Claeys; Bart Saerens; Ellen De Backer; Marleen Temmerman; Rita Verhelst; Mario Vaneechoutte
Journal:  BMC Infect Dis       Date:  2009-09-11       Impact factor: 3.090

10.  Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients.

Authors:  Pieter Deschaght; Thierry De Baere; Leen Van Simaey; Sabine Van Daele; Frans De Baets; Daniel De Vos; Jean-Paul Pirnay; Mario Vaneechoutte
Journal:  BMC Microbiol       Date:  2009-11-29       Impact factor: 3.605
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