Literature DB >> 9600926

Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel.

J le Coutre1, H R Kaback, C K Patel, L Heginbotham, C Miller.   

Abstract

The structure of the tetrameric K+ channel from Streptomyces lividans in a lipid bilayer environment was studied by polarized attenuated total reflection Fourier transform infrared spectroscopy. The channel displays approximately 43% alpha-helical and 25% beta-sheet content. In addition, H/D exchange experiments show that only 43% of the backbone amide protons are exchangeable with solvent. On average, the alpha-helices are tilted 33 degrees normal to the membrane surface. The results are discussed in relationship to the lactose permease of Escherichia coli, a membrane transport protein.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9600926      PMCID： PMC27594          DOI： 10.1073/pnas.95.11.6114

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  pH-dependent gating in the Streptomyces lividans K+ channel.

Authors:  L G Cuello; J G Romero; D M Cortes; E Perozo
Journal:  Biochemistry       Date:  1998-03-10       Impact factor: 3.162

Review 2.  Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins.

Authors:  S Krimm; J Bandekar
Journal:  Adv Protein Chem       Date:  1986

3.  Infrared spectroscopic study of photoreceptor membrane and purple membrane. Protein secondary structure and hydrogen deuterium exchange.

Authors:  N W Downer; T J Bruchman; J H Hazzard
Journal:  J Biol Chem       Date:  1986-03-15       Impact factor: 5.157

Review 4.  Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. I. Assignments and model compounds.

Authors:  E Goormaghtigh; V Cabiaux; J M Ruysschaert
Journal:  Subcell Biochem       Date:  1994

Review 5.  Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. III. Secondary structures.

Authors:  E Goormaghtigh; V Cabiaux; J M Ruysschaert
Journal:  Subcell Biochem       Date:  1994

6.  Potassium channels and their evolving gates.

Authors:  L Y Jan; Y N Jan
Journal:  Nature       Date:  1994-09-08       Impact factor: 49.962

7.  Agitoxin footprinting the shaker potassium channel pore.

Authors:  A Gross; R MacKinnon
Journal:  Neuron       Date:  1996-02       Impact factor: 17.173

8.  Determining the secondary structure and orientation of EmrE, a multi-drug transporter, indicates a transmembrane four-helix bundle.

Authors:  I T Arkin; W P Russ; M Lebendiker; S Schuldiner
Journal:  Biochemistry       Date:  1996-06-04       Impact factor: 3.162

9.  Silver as a probe of pore-forming residues in a potassium channel.

Authors:  Q Lü; C Miller
Journal:  Science       Date:  1995-04-14       Impact factor: 47.728

10.  A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans.

Authors:  H Schrempf; O Schmidt; R Kümmerlen; S Hinnah; D Müller; M Betzler; T Steinkamp; R Wagner
Journal:  EMBO J       Date:  1995-11-01       Impact factor: 11.598
View more
  38 in total

1.  Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins.

Authors:  J P Whitelegge; J le Coutre; J C Lee; C K Engel; G G Privé; K F Faull; H R Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  1999-09-14       Impact factor: 11.205

2.  Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane.

Authors:  Yiling Nie; H Ronald Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  2010-05-10       Impact factor: 11.205

3.  An approach to membrane protein structure without crystals.

Authors:  Paul L Sorgen; Yonglin Hu; Lan Guan; H Ronald Kaback; Mark E Girvin
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-21       Impact factor: 11.205

4.  Dynamics of a bacterial multidrug ABC transporter in the inward- and outward-facing conformations.

Authors:  Shahid Mehmood; Carmen Domene; Eric Forest; Jean-Michel Jault
Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-18       Impact factor: 11.205

5.  Positioning of proteins in membranes: a computational approach.

Authors:  Andrei L Lomize; Irina D Pogozheva; Mikhail A Lomize; Henry I Mosberg
Journal:  Protein Sci       Date:  2006-06       Impact factor: 6.725

Review 6.  Lessons from lactose permease.

Authors:  Lan Guan; H Ronald Kaback
Journal:  Annu Rev Biophys Biomol Struct       Date:  2006

7.  Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli.

Authors:  Yiling Nie; Irina Smirnova; Vladimir Kasho; H Ronald Kaback
Journal:  J Biol Chem       Date:  2006-09-26       Impact factor: 5.157

8.  FTIR spectroscopy of secondary-structure reorientation of melibiose permease modulated by substrate binding.

Authors:  Natàlia Dave; Víctor A Lórenz-Fonfría; Gérard Leblanc; Esteve Padrós
Journal:  Biophys J       Date:  2007-11-16       Impact factor: 4.033

9.  Sequence-specific conformational flexibility of SNARE transmembrane helices probed by hydrogen/deuterium exchange.

Authors:  Walter Stelzer; Bernhard C Poschner; Holger Stalz; Albert J Heck; Dieter Langosch
Journal:  Biophys J       Date:  2008-05-02       Impact factor: 4.033

10.  Protonation and sugar binding to LacY.

Authors:  Irina N Smirnova; Vladimir Kasho; H Ronald Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  2008-06-20       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.