Literature DB >> 9585501

Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis.

C Wang1, K Chua, W Seghezzi, E Lees, O Gozani, R Reed.   

Abstract

The U2 snRNP component SAP 155 contacts pre-mRNA on both sides of the branch site early in spliceosome assembly and is therefore positioned near or at the spliceosome catalytic center. We have isolated a cDNA encoding human SAP 155 and identified its highly related Saccharomyces cerevisiae homolog (50% identity). The carboxy-terminal two-thirds of SAP 155 shows the highest conservation and is remarkably similar to the regulatory subunit A of the phosphatase PP2A. Significantly, SAP 155 is phosphorylated concomitant with or just after catalytic step one, making this the first example of a protein modification tightly regulated with splicing catalysis.

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Year:  1998        PMID： 9585501      PMCID： PMC316838          DOI： 10.1101/gad.12.10.1409

Source DB:  PubMed          Journal:  Genes Dev        ISSN： 0890-9369            Impact factor:   11.361


  23 in total

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Authors:  J E Mermoud; P Cohen; A I Lamond
Journal:  Nucleic Acids Res       Date:  1992-10-25       Impact factor: 16.971

2.  Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at the splice sites.

Authors:  M J Moore; P A Sharp
Journal:  Science       Date:  1992-05-15       Impact factor: 47.728

Review 3.  Substrates for p34cdc2: in vivo veritas?

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Journal:  Cell       Date:  1990-05-18       Impact factor: 41.582

4.  Evidence that U5 snRNP recognizes the 3' splice site for catalytic step II in mammals.

Authors:  M D Chiara; L Palandjian; R Feld Kramer; R Reed
Journal:  EMBO J       Date:  1997-08-01       Impact factor: 11.598

5.  Molecular model of the A subunit of protein phosphatase 2A: interaction with other subunits and tumor antigens.

Authors:  R Ruediger; M Hentz; J Fait; M Mumby; G Walter
Journal:  J Virol       Date:  1994-01       Impact factor: 5.103

6.  Thiophosphorylation of U1-70K protein inhibits pre-mRNA splicing.

Authors:  J Tazi; U Kornstädt; F Rossi; P Jeanteur; G Cathala; C Brunel; R Lührmann
Journal:  Nature       Date:  1993-05-20       Impact factor: 49.962

7.  Correspondence between a mammalian spliceosome component and an essential yeast splicing factor.

Authors:  M Bennett; R Reed
Journal:  Science       Date:  1993-10-01       Impact factor: 47.728

8.  Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors.

Authors:  A Woppmann; C L Will; U Kornstädt; P Zuo; J L Manley; R Lührmann
Journal:  Nucleic Acids Res       Date:  1993-06-25       Impact factor: 16.971

9.  A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction.

Authors:  O Gozani; J G Patton; R Reed
Journal:  EMBO J       Date:  1994-07-15       Impact factor: 11.598

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Authors:  M B Roth; A M Zahler; J A Stolk
Journal:  J Cell Biol       Date:  1991-11       Impact factor: 10.539
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  94 in total

1.  Novel splice variants of cyclin E with altered substrate specificity.

Authors:  D C Porter; K Keyomarsi
Journal:  Nucleic Acids Res       Date:  2000-12-01       Impact factor: 16.971

2.  Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry.

Authors:  P Ajuh; B Kuster; K Panov; J C Zomerdijk; M Mann; A I Lamond
Journal:  EMBO J       Date:  2000-12-01       Impact factor: 11.598

3.  A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.

Authors:  C L Will; C Schneider; A M MacMillan; N F Katopodis; G Neubauer; M Wilm; R Lührmann; C C Query
Journal:  EMBO J       Date:  2001-08-15       Impact factor: 11.598

4.  Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation.

Authors:  D Nesic; A Krämer
Journal:  Mol Cell Biol       Date:  2001-10       Impact factor: 4.272

5.  Abscisic acid-induced transcription is mediated by phosphorylation of an abscisic acid response element binding factor, TRAB1.

Authors:  Yasuaki Kagaya; Tokunori Hobo; Michiharu Murata; Atushi Ban; Tsukaho Hattori
Journal:  Plant Cell       Date:  2002-12       Impact factor: 11.277

6.  Identification of a sequence element directing a protein to nuclear speckles.

Authors:  J Eilbracht; M S Schmidt-Zachmann
Journal:  Proc Natl Acad Sci U S A       Date:  2001-03-27       Impact factor: 11.205

7.  Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.

Authors:  Y Habara; S Urushiyama; T Shibuya; Y Ohshima; T Tani
Journal:  RNA       Date:  2001-05       Impact factor: 4.942

8.  Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast.

Authors:  David S McPheeters; Peggy Muhlenkamp
Journal:  Mol Cell Biol       Date:  2003-06       Impact factor: 4.272

9.  The FCP1 phosphatase interacts with RNA polymerase II and with MEP50 a component of the methylosome complex involved in the assembly of snRNP.

Authors:  Paolo Licciardo; Stefano Amente; Luca Ruggiero; Maria Monti; Piero Pucci; Luigi Lania; Barbara Majello
Journal:  Nucleic Acids Res       Date:  2003-02-01       Impact factor: 16.971

10.  Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus.

Authors:  Qiang Wang; Brian C Rymond
Journal:  Mol Cell Biol       Date:  2003-10       Impact factor: 4.272
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