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Literature DB >> 360168

3'End labelling of RNA with 32P suitable for rapid gel sequencing.

Abstract

A new general method of labelling the 2',3'-diol end of RNA with 32P has been devised suitable for gel sequencing. Poly(A) polymerase (E. coli) is incubated with the RNA and limiting amounts of alpha-32P-ATP. The mono-addition product is then cleaved with periodate and beta-eliminated with aniline, leaving the RNA terminally labelled with 3' 32P-phosphate. When applied to a model compound, tRNAPhe from E. coli, over 28 residues could be read from the 3' end.

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Year: 1978 PMID： 360168 PMCID： PMC342236 DOI： 10.1093/nar/5.9.3129

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

1. [DETERMINATION OF THE TRUE SUBSTRATE OF POLYNUCLEOTIDE PHOSPHORYLASE IN THE POLYMERIZATION OF ADP].

Authors: F R WILLIAMS; T GODEFROY; E MERY; J YON; M GRUNBERG-MANAGO
Journal: Biochim Biophys Acta Date: 1964-03-23

2. New rapid gel sequencing method for RNA.

Authors: A Simoncsits; G G Brownlee; R S Brown; J R Rubin; H Guilley
Journal: Nature Date: 1977-10-27 Impact factor: 49.962

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. Hydrolysis of poly (A) to adenine nucleotides by purified poly (A) polymerase.

Authors: A K Abraham; S T Jacob
Journal: Proc Natl Acad Sci U S A Date: 1978-05 Impact factor: 11.205

5. Enzymes for RNA sequence analysis. Preparation and specificity of exoplasmodial ribonucleases I and II from Physarum polycephalum.

Authors: D Pilly; A Niemeyer; M Schmidt; J P Bargetzi
Journal: J Biol Chem Date: 1978-01-25 Impact factor: 5.157

6. Terminal riboadenylate transferase from Escherichia coli. Characterization and application.

Authors: H Sano; G Feix
Journal: Eur J Biochem Date: 1976-12-11

7. The use of thin acrylamide gels for DNA sequencing.

Authors: F Sanger; A R Coulson
Journal: FEBS Lett Date: 1978-03-01 Impact factor: 4.124

8. Rapid gel sequencing of RNA by primed synthesis with reverse transcriptase.

Authors: G G Brownlee; E M Cartwright
Journal: J Mol Biol Date: 1977-07 Impact factor: 5.469

9. TERMINAL-SEQUENCE STUDIES OF HIGH-MOLECULAR-WEIGHT RIBONUCLEIC. THE REACTION OF PERIODATE-OXIDIZED RIBONUCLEOSIDES , 5'-RIBONUCLEOTIDES AND RIBONUCLEIC ACID WITH ISONIAZID.

Authors: J A HUNT
Journal: Biochem J Date: 1965-05 Impact factor: 3.857

10. Mapping adenines, guanines, and pyrimidines in RNA.

Authors: H Donis-Keller; A M Maxam; W Gilbert
Journal: Nucleic Acids Res Date: 1977-08 Impact factor: 16.971

16 in total

1. A family of wheat embryo U2 snRNAs.

Authors: J M Skuzeski; J J Jendrisak
Journal: Plant Mol Biol Date: 1985-03 Impact factor: 4.076

2. The electrophoretic analysis of low molecular weight nucleic acids from Crohn's disease tissues in the search for an unconventional small infectious agent. Brief report.

Authors: P D Butcher; J J McFadden; J Hermon-Taylor
Journal: Arch Virol Date: 1986 Impact factor: 2.574

3. Specificity for 3',5'-linked substrates in RNA-catalyzed RNA polymerization.

Authors: B Young; T R Cech
Journal: J Mol Evol Date: 1989-12 Impact factor: 2.395

4. Chloroplast-like transfer RNA genes expressed in wheat mitochondria.

Authors: P B Joyce; M W Gray
Journal: Nucleic Acids Res Date: 1989-07-25 Impact factor: 16.971

5. Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides.

Authors: G Martin; W Keller
Journal: RNA Date: 1998-02 Impact factor: 4.942

6. Primary and secondary structure of dinoflagellate U5 small nuclear RNA.

Authors: M H Liu; R Reddy; D Henning; D Spector; H Busch
Journal: Nucleic Acids Res Date: 1984-02-10 Impact factor: 16.971

7. T4 RNA ligase catalyzed synthesis of base analogue-containing oligodeoxyribonucleotides and a characterization of their thermal stabilities.

Authors: C A Brennan; R I Gumport
Journal: Nucleic Acids Res Date: 1985-12-20 Impact factor: 16.971