Literature DB >> 954089

A direct estimate of the number of human gamma-globin genes.

J Old, J B Clegg, D J Weatherall, S Ottolenghi, P Comi, B Giglioni, J Mitchell, P Tolstoshev, R Williamson.   

Abstract

The number of genes specifying human gamma-globin has been determined directly by hybridization of complementary DNA to total human DNA. The complementary DNA was enriched in sequences specific for gamma-globin genes by transcribing globin mRNA isolated from fetal reticulocytes with viral reverse transcriptase, and collecting the material which does not back-hybridize to adult globin mRNA. When hybridized in cDNA excess to DNA, very similar values are found for gamma-gene number as for beta-gene number, suggesting two or at most three gamma-globin genes per haploid human genome. This indicates that the non-Mendelian ratios of gamma-chain mutants found in heterozygotes are due to transcriptional or post-transcriptional regulation rather than to gene dosage. The number of each major human globin gene has now been determined directly by molecular methods.

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Year:  1976        PMID： 954089     DOI： 10.1016/0092-8674(76)90180-x

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  11 in total

1.  Quantitation of human gamma globin genes and gamma globin mRNA with purified gamma globin complementary DNA.

Authors:  F Ramirez; J V O'Donnell; C Natta; A Bank
Journal:  J Clin Invest       Date:  1976-12       Impact factor: 14.808

2.  Purification of cDNA complementary to sea urchin histone mRNA.

Authors:  D Woods; W Fitschen
Journal:  Nucleic Acids Res       Date:  1977-09       Impact factor: 16.971

3.  The chemical heterogeneity of the fetal hemoglobin in normal newborn infants and in adults.

Authors:  T H Huisman; H Harris; M Gravely
Journal:  Mol Cell Biochem       Date:  1977-08-19       Impact factor: 3.396

4.  Restriction endonuclease mapping of the human gamma globin gene loci.

Authors:  D Tuan; P A Biro; J K deRiel; H Lazarus; B G Forget
Journal:  Nucleic Acids Res       Date:  1979-06-11       Impact factor: 16.971

5.  Defects in DNA and globin messenger RNA in homozygotes for hemoglobin Lepore.

Authors:  F Ramirez; J G Mears; U Nudel; A Bank; L Luzzatto; G DiPrisco; R D'Avino; G Pepe; L Camardella; R Gambino; R Cimino; N Quattrin
Journal:  J Clin Invest       Date:  1979-04       Impact factor: 14.808

6.  Genes for gamma-globin in human adult erythroid DNA.

Authors:  G J Mitchell; R Williamson
Journal:  Nucleic Acids Res       Date:  1977-10       Impact factor: 16.971

7.  Preparation of a complementary DNA for leghaemoglobin and direct demonstration that leghaemoglobin is encoded by the soybean genome.

Authors:  D Baulcombe; D P Verma
Journal:  Nucleic Acids Res       Date:  1978-11       Impact factor: 16.971

8.  Restriction mapping of a new deletion responsible for G gamma (delta beta)o thalassemia.

Authors:  R W Jones; J M Old; R J Trent; J B Clegg; D J Weatherall
Journal:  Nucleic Acids Res       Date:  1981-12-21       Impact factor: 16.971

9.  Human T gamma globin chain is a variant of A gamma chain (A gamma Sardinia).

Authors:  G Saglio; G Ricco; U Mazza; C Camaschella; P G Pich; A M Gianni; E Gianazza; P G Righetti; B Giglioni; P Comi; M Gusmeroli; S Ottolenghi
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

10.  Number and location of mouse mammary tumor virus proviral DNA in mouse DNA of normal tissue and of mammary tumors.

Authors:  B Groner; N E Hynes
Journal:  J Virol       Date:  1980-03       Impact factor: 5.103
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