Literature DB >> 909803

Purification of cDNA complementary to sea urchin histone mRNA.

D Woods, W Fitschen.   

Abstract

Complementary DNA (cDNA) was transcribed from a polyadenylated sea urchin histone mRNA preparation isolated by density gradient centrifugation. By hybridization, this cDNA was shown to be extensively contaminated (85% of hybridizable cDNA) with DNA complementary to RNA derived from the large ribosomal subunit. Purification of a mRNA specific cDNA fraction was achieved by hybridization of purified rRNA to cDNA followed by fractionation on hydroxyapatite. After further purification to remove nonhybridizable cDNA our purified cDNA showed only 8% hybirdization to rRNA.

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Year:  1977        PMID： 909803      PMCID： PMC342643          DOI： 10.1093/nar/4.9.3187

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  32 in total

1.  The organization of sea urchin histone genes.

Authors:  L H Kedes; R H Cohn; J C Lowry; A C Chang; S N Cohen
Journal:  Cell       Date:  1975-11       Impact factor: 41.582

2.  Partial purification and characterization of individual histone messenger RNAs from HeLa cells.

Authors:  E Bos; W Roskam; D Gallwitz
Journal:  Biochem Biophys Res Commun       Date:  1976-11-22       Impact factor: 3.575

3.  Characterization of a mouse ascites cell-free system.

Authors:  M Jacobs-Lorena; C Baglioni
Journal:  Biochemistry       Date:  1972-12-19       Impact factor: 3.162

4.  Kinetic studies of gene frequency. II. Complexity of globin complementary DNA and its hybridization characteristics.

Authors:  B D Young; P R Harrison; R S Gilmour; G D Birnie; A Hell; S Humphries; J Paul
Journal:  J Mol Biol       Date:  1974-04-25       Impact factor: 5.469

5.  Kinetic studies of gene frequency. I. Use of a DNA copy of reticulocyte 9 S RNA to estimate globin gene dosage in mouse tissues.

Authors:  P R Harrison; G D Birnie; A Hell; S Humphries; B D Young; J Paul
Journal:  J Mol Biol       Date:  1974-04-25       Impact factor: 5.469

6.  Cell-free synthesis of histones directed by messenger RNA from sea urchin embryos.

Authors:  K Gross; J Ruderman; M Jacobs-Lorena; C Baglioni; P R Gross
Journal:  Nat New Biol       Date:  1973-02-28

7.  Reiteration and clustering of DNA sequences complementary to histone messenger RNA.

Authors:  L H Kedes; M L Birnstiel
Journal:  Nat New Biol       Date:  1971-04-07

8.  The relative positions of sea urchin histone genes on the chimeric plasmids pSp2 and pSp17 as studied by electronmicroscopy.

Authors:  M Wu; D S Holmes; N Davidson; R H Cohn; L H Kedes
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

9.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

10.  Histone genes of the sea urchin (S. purpuratus) cloned in E coli: order, polarity, and strandedness of the five histone-coding and spacer regions.

Authors:  R H Cohn; J C Lowry; L H Kedes
Journal:  Cell       Date:  1976-09       Impact factor: 41.582
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  2 in total

1.  Globin gene expression in MSV-transformed fibroblasts.

Authors:  I Parker; W Fitschen
Journal:  Experientia       Date:  1979-10-15

2.  Procollagen mRNA metabolism during the fibroblast cell cycle and its synthesis in transformed cells.

Authors:  I Parker; W Fitschen
Journal:  Nucleic Acids Res       Date:  1980-06-25       Impact factor: 16.971
  2 in total

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