Literature DB >> 9473461

Human placental alkaline phosphatase: expression in Pichia pastoris, purification and characterization of the enzyme.

H Heimo1, K Palmu, I Suominen.   

Abstract

The soluble form of human placental alkaline phosphatase (PLAP) was expressed in the methylotrophic yeast Pichia pastoris and the expression product was purified and characterized. Yeast-derived PLAP (yPLAP) was secreted into the medium to the level of 2 mg/liter. yPLAP displayed kinetic properties similar to those reported earlier for the membrane-bound PLAP. Purified yPLAP had specific activity of 774 U/mg and appeared in two subunit sizes, ca. 62 and 65 kDa. This difference was due to heterogenous N-glycosylation. Purified yPLAP appeared as multiple forms in isoelectric focusing in pI range of 4.2 to 5.2. The expression system is discussed in comparison to previously reported expression systems. Copyright 1998 Academic Press.

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Year:  1998        PMID： 9473461     DOI： 10.1006/prep.1997.0808

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  5 in total

1.  Cloning and expression of an anti-LDL(-) single-chain variable fragment, and its inhibitory effect on experimental atherosclerosis.

Authors:  Soraya M Kazuma; Marcela F Cavalcante; Andréia E R Telles; Andrea Queiroz Maranhão; Dulcineia S P Abdalla
Journal:  MAbs       Date:  2013-07-25       Impact factor: 5.857

Review 2.  Recombinant protein expression in Pichia pastoris.

Authors:  J M Cregg; J L Cereghino; J Shi; D R Higgins
Journal:  Mol Biotechnol       Date:  2000-09       Impact factor: 2.860

3.  Kinetic comparison of tissue non-specific and placental human alkaline phosphatases expressed in baculovirus infected cells: application to screening for Down's syndrome.

Authors:  Colette C Denier; Andrée A Brisson-Lougarre; Ghislaine G Biasini; Jean J Grozdea; Didier D Fournier
Journal:  BMC Biochem       Date:  2002-01-15       Impact factor: 4.059

4.  Bioproduced Proteins On Demand (Bio-POD) in hydrogels using Pichia pastoris.

Authors:  Shuo-Fu Yuan; Sierra M Brooks; Annalee W Nguyen; Wen-Ling Lin; Trevor G Johnston; Jennifer A Maynard; Alshakim Nelson; Hal S Alper
Journal:  Bioact Mater       Date:  2021-01-27

5.  A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter.

Authors:  Ferez S Nallaseth; Stephen Anderson
Journal:  Microb Cell Fact       Date:  2013-04-19       Impact factor: 5.328
  5 in total

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