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Literature DB >> 9469829

Codon-based mutagenesis using dimer-phosphoramidites.

Abstract

A new approach for the synthesis of randomized DNA sequences containing the 20 codons corresponding to all natural amino acids is described. The strategy is based on the use of dinucleotide phosphoramidite building blocks within a resin-splitting procedure. Through this protocol, a minimal number of seven dimers is sufficient to encode all 20 natural amino acids. This synthesis procedure is extremely flexible and allows codon usage from different hosts to be accommodated.

Entities: Chemical

Mesh：

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Year: 1998 PMID： 9469829 PMCID： PMC147399 DOI： 10.1093/nar/26.5.1223

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

11 in total

1. A new type of synthetic peptide library for identifying ligand-binding activity.

Authors: K S Lam; S E Salmon; E M Hersh; V J Hruby; W M Kazmierski; R J Knapp
Journal: Nature Date: 1991-11-07 Impact factor: 49.962

2. A convenient approach to the synthesis of trinucleotide phosphoramidites--synthons for the generation of oligonucleotide/peptide libraries.

Authors: A L Kayushin; M D Korosteleva; A I Miroshnikov; W Kosch; D Zubov; N Piel
Journal: Nucleic Acids Res Date: 1996-10-01 Impact factor: 16.971

3. Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

Authors: B Virnekäs; L Ge; A Plückthun; K C Schneider; G Wellnhofer; S E Moroney
Journal: Nucleic Acids Res Date: 1994-12-25 Impact factor: 16.971

4. Identification of biologically active mutants by combinatorial cassette mutagenesis: exclusion of wild-type codon from degenerate codons.

Authors: W Huang; D V Santi
Journal: Anal Biochem Date: 1994-05-01 Impact factor: 3.365

5. A general strategy for random insertion and substitution mutagenesis: substoichiometric coupling of trinucleotide phosphoramidites.

Authors: J Sondek; D Shortle
Journal: Proc Natl Acad Sci U S A Date: 1992-04-15 Impact factor: 11.205

Codon-based mutagenesis using dimer-phosphoramidites.

1. A new type of synthetic peptide library for identifying ligand-binding activity.

2. A convenient approach to the synthesis of trinucleotide phosphoramidites--synthons for the generation of oligonucleotide/peptide libraries.

3. Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

4. Identification of biologically active mutants by combinatorial cassette mutagenesis: exclusion of wild-type codon from degenerate codons.

5. A general strategy for random insertion and substitution mutagenesis: substoichiometric coupling of trinucleotide phosphoramidites.

6. Construction of a semisynthetic antibody library using trinucleotide oligos.

7. Regional codon randomization: defining a TATA-binding protein surface required for RNA polymerase III transcription.

8. The synthesis of blocked triplet-phosphoramidites and their use in mutagenesis.

9. Mutagenesis using trinucleotide beta-cyanoethyl phosphoramidites.

10. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.

1. Protein evolution by codon-based random deletions.

2. In vivo selection of protease cleavage sites by using chimeric Sindbis virus libraries.

3. Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection.

4. Biomathematical description of synthetic peptide libraries.

5. Economical analysis of saturation mutagenesis experiments.

6. TrimerDimer: an oligonucleotide-based saturation mutagenesis approach that removes redundant and stop codons.

7. Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries.

Review 8. Evolving a Peptide: Library Platforms and Diversification Strategies.