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Literature DB >> 9358184

Construction of a semisynthetic antibody library using trinucleotide oligos.

Abstract

A semisynthetic antibody library composed of single chain Fv fragments (scFv) was constructed by replacing the heavy chain CDR3 region of a human scFv by a random sequence of eight amino acids using trinucleotide codons. After cloning into a phage display vector, an antibody library was generated with a complexity of 8 x 10(8) independent clones. The library was screened for binders to dinitrophenol, fluorescein isothiocyanate and 3-nitro-4-hydroxy-5-iodophenylacetic acid. scFv antibodies that specifically bound the antigen were obtained in each case.

Entities: Chemical Gene Species

Mesh：

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Year: 1997 PMID： 9358184 PMCID： PMC147064 DOI： 10.1093/nar/25.22.4690

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

9 in total

1. A convenient approach to the synthesis of trinucleotide phosphoramidites--synthons for the generation of oligonucleotide/peptide libraries.

Authors: A L Kayushin; M D Korosteleva; A I Miroshnikov; W Kosch; D Zubov; N Piel
Journal: Nucleic Acids Res Date: 1996-10-01 Impact factor: 16.971

2. Construction of a human Ig combinatorial library from genomic V segments and synthetic CDR3 fragments.

Authors: Y Akamatsu; M S Cole; J Y Tso; N Tsurushita
Journal: J Immunol Date: 1993-11-01 Impact factor: 5.422

3. The antigen-binding domain of a human IgG-anti-F(ab')2 autoantibody.

Authors: M Welschof; P Terness; S M Kipriyanov; D Stanescu; F Breitling; H Dörsam; S Dübel; M Little; G Opelz
Journal: Proc Natl Acad Sci U S A Date: 1997-03-04 Impact factor: 11.205

4. Making antibody fragments using phage display libraries.

Authors: T Clackson; H R Hoogenboom; A D Griffiths; G Winter
Journal: Nature Date: 1991-08-15 Impact factor: 49.962

5. Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions.

Authors: J de Kruif; E Boel; T Logtenberg
Journal: J Mol Biol Date: 1995-04-21 Impact factor: 5.469

Review 6. Human antibody libraries in Escherichia coli.

Authors: M Little; F Breitling; S Dübel; P Fuchs; M Braunagel
Journal: J Biotechnol Date: 1995-07-31 Impact factor: 3.307

7. By-passing immunization: building high affinity human antibodies by chain shuffling.

Authors: J D Marks; A D Griffiths; M Malmqvist; T P Clackson; J M Bye; G Winter
Journal: Biotechnology (N Y) Date: 1992-07

8. By-passing immunization. Human antibodies from V-gene libraries displayed on phage.

Authors: J D Marks; H R Hoogenboom; T P Bonnert; J McCafferty; A D Griffiths; G Winter
Journal: J Mol Biol Date: 1991-12-05 Impact factor: 5.469

9. Antibody fragments from a 'single pot' phage display library as immunochemical reagents.

Authors: A Nissim; H R Hoogenboom; I M Tomlinson; G Flynn; C Midgley; D Lane; G Winter
Journal: EMBO J Date: 1994-02-01 Impact factor: 11.598

9 in total

11 in total

Review 1. Generation of recombinant antibodies.

Authors: S M Kipriyanov; M Little
Journal: Mol Biotechnol Date: 1999-09 Impact factor: 2.695

2. Heteroduplex mobility assay (HMA) pre-screening: an improved strategy for the rapid identification of inserts selected from phage-displayed peptide libraries.

Authors: F Fack; S Deroo; S Kreis; C P Muller
Journal: Mol Divers Date: 2000 Impact factor: 2.943

Construction of a semisynthetic antibody library using trinucleotide oligos.

1. A convenient approach to the synthesis of trinucleotide phosphoramidites--synthons for the generation of oligonucleotide/peptide libraries.

2. Construction of a human Ig combinatorial library from genomic V segments and synthetic CDR3 fragments.

3. The antigen-binding domain of a human IgG-anti-F(ab')2 autoantibody.

4. Making antibody fragments using phage display libraries.

5. Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions.

Review 6. Human antibody libraries in Escherichia coli.

7. By-passing immunization: building high affinity human antibodies by chain shuffling.

8. By-passing immunization. Human antibodies from V-gene libraries displayed on phage.

9. Antibody fragments from a 'single pot' phage display library as immunochemical reagents.

Review 1. Generation of recombinant antibodies.

2. Heteroduplex mobility assay (HMA) pre-screening: an improved strategy for the rapid identification of inserts selected from phage-displayed peptide libraries.

Review 3. Recombinant antibodies for the diagnosis and treatment of cancer.

4. Understanding differences between synthetic and natural antibodies can help improve antibody engineering.

5. Codon-based mutagenesis using dimer-phosphoramidites.

6. A single donor is sufficient to produce a highly functional in vitro antibody library.

7. Natural and man-made V-gene repertoires for antibody discovery.

Review 8. Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis.

9. Covalent antibody display--an in vitro antibody-DNA library selection system.

10. Many Routes to an Antibody Heavy-Chain CDR3: Necessary, Yet Insufficient, for Specific Binding.