Literature DB >> 7838712

Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

B Virnekäs1, L Ge, A Plückthun, K C Schneider, G Wellnhofer, S E Moroney.   

Abstract

Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

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Year:  1994        PMID： 7838712      PMCID： PMC310122          DOI： 10.1093/nar/22.25.5600

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

Review 1.  New recombinant DNA methodology for protein engineering.

Authors:  M J Zoller
Journal:  Curr Opin Biotechnol       Date:  1992-08       Impact factor: 9.740

2.  Optimizing nucleotide mixtures to encode specific subsets of amino acids for semi-random mutagenesis.

Authors:  A P Arkin; D C Youvan
Journal:  Biotechnology (N Y)       Date:  1992-03

3.  Antibody engineering by codon-based mutagenesis in a filamentous phage vector system.

Authors:  S M Glaser; D E Yelton; W D Huse
Journal:  J Immunol       Date:  1992-12-15       Impact factor: 5.422

4.  Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites.

Authors:  J A Wells; M Vasser; D B Powers
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  A general strategy for random insertion and substitution mutagenesis: substoichiometric coupling of trinucleotide phosphoramidites.

Authors:  J Sondek; D Shortle
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

6.  Preparation of oligodeoxyribonucleoside phosphorodithioates by a triester method.

Authors:  A B Eldrup; K Bjergårde; J Felding; J Kehler; O Dahl
Journal:  Nucleic Acids Res       Date:  1994-05-25       Impact factor: 16.971

7.  Codon usage and gene expression.

Authors:  L Holm
Journal:  Nucleic Acids Res       Date:  1986-04-11       Impact factor: 16.971
  7 in total
  56 in total

Review 1.  Generation of recombinant antibodies.

Authors:  S M Kipriyanov; M Little
Journal:  Mol Biotechnol       Date:  1999-09       Impact factor: 2.695

2.  Rapid mapping of protein functional epitopes by combinatorial alanine scanning.

Authors:  G A Weiss; C K Watanabe; A Zhong; A Goddard; S S Sidhu
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-01       Impact factor: 11.205

3.  Construction and characterization of protein libraries composed of secondary structure modules.

Authors:  Tomoaki Matsuura; Andreas Ernst; Andreas Plückthun
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

4.  Optimizing doped libraries by using genetic algorithms.

Authors:  D Tomandl; A Schober; A Schwienhorst
Journal:  J Comput Aided Mol Des       Date:  1997-01       Impact factor: 3.686

5.  Combinatorial redesign of the DNA binding specificity of a prokaryotic helix-turn-helix repressor.

Authors:  Katja Fromknecht; Pia D Vogel; John G Wise
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

6.  Designed to be stable: crystal structure of a consensus ankyrin repeat protein.

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7.  Strategies for selection from protein libraries composed of de novo designed secondary structure modules.

Authors:  Tomoaki Matsuura; Andreas Plückthun
Journal:  Orig Life Evol Biosph       Date:  2004-02       Impact factor: 1.950

8.  General strategy for the generation of human antibody variable domains with increased aggregation resistance.

Authors:  Kip Dudgeon; Romain Rouet; Iris Kokmeijer; Peter Schofield; Jessica Stolp; David Langley; Daniela Stock; Daniel Christ
Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-27       Impact factor: 11.205

9.  Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection.

Authors:  Paul Gaytán; Joel Osuna; Xavier Soberón
Journal:  Nucleic Acids Res       Date:  2002-08-15       Impact factor: 16.971

10.  In vitro evolution of enzymes.

Authors:  Misha V Golynskiy; John C Haugner; Aleardo Morelli; Dana Morrone; Burckhard Seelig
Journal:  Methods Mol Biol       Date:  2013
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