Literature DB >> 9464424

Construction of a sorbitol-based vector for expression of heterologous proteins in Saccharomyces cerevisiae.

T McGonigal1, P Bodelle, C Schopp, A V Sarthy.   

Abstract

A new inducible yeast expression vector, pXS7, was constructed by using the promoter and terminator sequences from the Saccharomyces cerevisiae SOR1 gene, which codes for the sorbitol dehydrogenase protein. We cloned the coding sequence of the Saccharomyces YEF3 gene in this vector and demonstrated an increase in YEF3 protein levels when cells were grown in the presence of the sugar sorbitol.

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Year:  1998        PMID: 9464424      PMCID: PMC106122     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  7 in total

1.  Improved method for high efficiency transformation of intact yeast cells.

Authors:  D Gietz; A St Jean; R A Woods; R H Schiestl
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

2.  Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae.

Authors:  J O Mascorro-Gallardo; A A Covarrubias; R Gaxiola
Journal:  Gene       Date:  1996-06-12       Impact factor: 3.688

3.  Synthesis and assembly of hepatitis B virus surface antigen particles in yeast.

Authors:  P Valenzuela; A Medina; W J Rutter; G Ammerer; B D Hall
Journal:  Nature       Date:  1982-07-22       Impact factor: 49.962

4.  Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

Authors:  M Johnston; R W Davis
Journal:  Mol Cell Biol       Date:  1984-08       Impact factor: 4.272

5.  Selection of functional cDNAs by complementation in yeast.

Authors:  G L McKnight; B L McConaughy
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

6.  Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae.

Authors:  A V Sarthy; C Schopp; K B Idler
Journal:  Gene       Date:  1994-03-11       Impact factor: 3.688

7.  Expression of hepatitis B virus surface antigen in yeast.

Authors:  R A Hitzeman; C Y Chen; F E Hagie; E J Patzer; C C Liu; D A Estell; J V Miller; A Yaffe; D G Kleid; A D Levinson; H Oppermann
Journal:  Nucleic Acids Res       Date:  1983-05-11       Impact factor: 16.971

  7 in total
  2 in total

1.  Analysis of Xylose Operon from Paenibacillus polymyxa ATCC842 and Development of Tools for Gene Expression.

Authors:  Zilong Wang; Yakun Fang; Yi Shi; Yu Xin; ZhengHua Gu; Ting Yang; Youran Li; Zhongyang Ding; Guiyang Shi; Liang Zhang
Journal:  Int J Mol Sci       Date:  2022-04-30       Impact factor: 6.208

2.  pBT, a novel vector for tetracycline-regulated yeast three-hybrid assay.

Authors:  Koki Moriyoshi
Journal:  Nucleic Acids Res       Date:  2008-12-02       Impact factor: 16.971

  2 in total

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