Literature DB >> 8654982

Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae.

J O Mascorro-Gallardo1, A A Covarrubias, R Gaxiola.   

Abstract

We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.

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Year:  1996        PMID： 8654982     DOI： 10.1016/0378-1119(96)00059-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  26 in total

1.  The CUP1 upstream repeated element renders CUP1 promoter activation insensitive to mutations in the RNA polymerase II transcription complex.

Authors:  Laura Badi; Alcide Barberis
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

2.  Regulatable killing of eukaryotic cells by the prokaryotic proteins Kid and Kis.

Authors:  Guillermo de la Cueva-Méndez; Anthony D Mills; Lorena Clay-Farrace; Ramón Díaz-Orejas; Ronald A Laskey
Journal:  EMBO J       Date:  2003-01-15       Impact factor: 11.598

3.  Increased copper bioremediation ability of new transgenic and adapted Saccharomyces cerevisiae strains.

Authors:  Polina Geva; Rotem Kahta; Faina Nakonechny; Stella Aronov; Marina Nisnevitch
Journal:  Environ Sci Pollut Res Int       Date:  2016-07-08       Impact factor: 4.223

4.  A new function in translocation for the mitochondrial i-AAA protease Yme1: import of polynucleotide phosphorylase into the intermembrane space.

Authors:  Robert N Rainey; Jenny D Glavin; Hsiao-Wen Chen; Samuel W French; Michael A Teitell; Carla M Koehler
Journal:  Mol Cell Biol       Date:  2006-09-11       Impact factor: 4.272

5.  Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system.

Authors:  K Siegers; T Waldmann; M R Leroux; K Grein; A Shevchenko; E Schiebel; F U Hartl
Journal:  EMBO J       Date:  1999-01-04       Impact factor: 11.598

6.  Global and specific translational regulation in the genomic response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source.

Authors:  K M Kuhn; J L DeRisi; P O Brown; P Sarnow
Journal:  Mol Cell Biol       Date:  2001-02       Impact factor: 4.272

7.  Construction of a sorbitol-based vector for expression of heterologous proteins in Saccharomyces cerevisiae.

Authors:  T McGonigal; P Bodelle; C Schopp; A V Sarthy
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

8.  Internal initiation in Saccharomyces cerevisiae mediated by an initiator tRNA/eIF2-independent internal ribosome entry site element.

Authors:  S R Thompson; K D Gulyas; P Sarnow
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-30       Impact factor: 11.205

9.  Translation initiation factors are not required for Dicistroviridae IRES function in vivo.

Authors:  Nilsa Deniz; Erik M Lenarcic; Dori M Landry; Sunnie R Thompson
Journal:  RNA       Date:  2009-03-19       Impact factor: 4.942

10.  Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus.

Authors:  David B Kushner; Brett D Lindenbach; Valery Z Grdzelishvili; Amine O Noueiry; Scott M Paul; Paul Ahlquist
Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-11       Impact factor: 11.205
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