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Literature DB >> 939761

Enzyme coupled immunoassay of insulin using a novel coupling reagent.

Abstract

A novel coupling reagent, meta-maleimidobenzoyl N-hydroxysuccinimide ester was synthesized. Using this reagent, insulin was conjugated very easily with beta-D-galactosidase [EC 3.2.1.23] in neutral, aqueous solution. No reduction of the enzyme activity was observed during the coupling procedure. The competitive bindings of the conjugate and insulin to anti-insulin serum were tested. The results indicated that the conjugate has enough immune reactivity for use in enzyme coupled immunoassay. Using this assay 20-800 pg of insulin were detectable.

Entities: Chemical Gene

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Year: 1976 PMID： 939761 DOI： 10.1093/oxfordjournals.jbchem.a131053

Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387

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Cited

48 in total

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4. Epitope mapping of B-cell determinants on the 15-kilodalton lipoprotein of Treponema pallidum (Tpp15) with synthetic peptides.

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5. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein.

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6. Electrophoretic heterogeneity limits the utility of streptavidin-β-galactosidase as a probe in free zone capillary electrophoresis separations.

Authors: Douglas B Craig; Anna Henderson
Journal: Protein J Date: 2013-02 Impact factor: 2.371

Enzyme coupled immunoassay of insulin using a novel coupling reagent.

1. A family of beta 7 integrins on human mucosal lymphocytes.

2. Alternatively spliced platelet-derived growth factor A-chain transcripts are not tumor specific but encode normal cellular proteins.

3. Bi-specific MHC heterodimers for characterization of cross-reactive T cells.

4. Epitope mapping of B-cell determinants on the 15-kilodalton lipoprotein of Treponema pallidum (Tpp15) with synthetic peptides.

5. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein.

6. Electrophoretic heterogeneity limits the utility of streptavidin-β-galactosidase as a probe in free zone capillary electrophoresis separations.

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