Literature DB >> 2497137

Production of antibody to and cellular localization of erythrocyte-sensitizing substance from Rickettsia rickettsii.

K E Hechemy1, J Fox, W A Samsonoff, R Anacker, D Silverman, C Eisemann, I S Green.   

Abstract

Antibodies to Rickettsia rickettsii erythrocyte-sensitizing substance (ESS) were raised in rabbits by using a derivatized ESS. The resulting antibodies reacted with R. rickettsii and cross-reacted with Rickettsia conorii, a member of the spotted fever group rickettsiae, but did not react with Rickettsia typhi, a member of the typhus group rickettsiae, Legionella bozemanii, or Proteus vulgaris OX19 or OX2. Immunoblot analysis indicated that ESS was present in more than one fraction and that the major haptenic fraction was proteinase resistant. Immunoelectron microscopy indicated that the antibodies to R. rickettsii were specific to components located on the cell surface and intracellularly to components between the cell wall and cytoplasmic membrane.

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Year:  1989        PMID： 2497137      PMCID： PMC267325          DOI： 10.1128/jcm.27.3.377-384.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  34 in total

1.  Factors affecting the growth of rickettsias of the spotted fever group in fertile hens' eggs.

Authors:  H G STOENNER; D B LACKMAN; E J BELL
Journal:  J Infect Dis       Date:  1962 Mar-Apr       Impact factor: 5.226

2.  Morphological, chemical, and serological studies of the cell walls of Rickettsia mooseri.

Authors:  M SCHAECHTER; A J TOUSIMIS; Z A COHN; H ROSEN; J CAMPBELL; F E HAHN
Journal:  J Bacteriol       Date:  1957-12       Impact factor: 3.490

3.  Enzyme coupled immunoassay of insulin using a novel coupling reagent.

Authors:  T Kitagawa; T Aikawa
Journal:  J Biochem       Date:  1976-01       Impact factor: 3.387

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Studies of Rickettsia mooseri cell walls. II. Immunologic properties.

Authors:  W H Wood; C L Wisseman
Journal:  J Immunol       Date:  1967-06       Impact factor: 5.422

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation.

Authors:  E Weiss; J C Coolbaugh; J C Williams
Journal:  Appl Microbiol       Date:  1975-09

8.  A comparison of serologic methods for diagnosis of Rocky Mountain spotted fever.

Authors:  R N Philip; E A Casper; J N MacCormack; D Sexton; L A Thomas; R L Anacker; W Burgdorfer; S Vick
Journal:  Am J Epidemiol       Date:  1977-01       Impact factor: 4.897

9.  The preparation of antigenic hapten-carrier conjugates: a survey.

Authors:  B F Erlanger
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

10.  External layers of Rickettsia prowazekii and Rickettsia rickettsii: occurrence of a slime layer.

Authors:  D J Silverman; C L Wisseman; A D Waddell; M Jones
Journal:  Infect Immun       Date:  1978-10       Impact factor: 3.441
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  5 in total

1.  Immunoblot studies to analyze antibody to the Rickettsia typhi group antigen in sera from patients with acute febrile cerebrovasculitis.

Authors:  K E Hechemy; J A Fox; D H Gröschel; F G Hayden; R P Wenzel
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

2.  Evaluation of the high-density agglutination test for Coxiella burnetii antibodies in animals.

Authors:  S V Nguyen; H To; N Minamoto; M Ogawa; T Yamaguchi; H Fukushi; K Hirai
Journal:  Clin Diagn Lab Immunol       Date:  1997-11

3.  Rapid method for detection of Coxiella burnetii antibodies using high-density particle agglutination.

Authors:  S V Nguyen; H Otsuka; G Q Zhang; H To; T Yamaguchi; H Fukushi; A Noma; K Hirai
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

4.  Enzyme-linked immunosorbent assay for detection of human immunoglobulin G to lipopolysaccharide of spotted fever group rickettsiae.

Authors:  D Jones; B Anderson; J Olson; C Greene
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948

5.  Comparative evaluation of a commercial enzyme immunoassay for the detection of human antibody to Rickettsia typhi.

Authors:  D J Kelly; C T Chan; H Paxton; K Thompson; R Howard; G A Dasch
Journal:  Clin Diagn Lab Immunol       Date:  1995-05
  5 in total

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