Literature DB >> 9380524

Betaine improves the PCR amplification of GC-rich DNA sequences.

W Henke1, K Herdel, K Jung, D Schnorr, S A Loening.   

Abstract

Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.

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Year:  1997        PMID： 9380524      PMCID： PMC146979          DOI： 10.1093/nar/25.19.3957

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  Formamide can dramatically improve the specificity of PCR.

Authors:  G Sarkar; S Kapelner; S S Sommer
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

2.  A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes.

Authors:  B P Sokolov; D J Prockop
Journal:  Nucleic Acids Res       Date:  1994-09-25       Impact factor: 16.971

3.  Optimizing multiplex and LA-PCR with betaine.

Authors:  P N Hengen
Journal:  Trends Biochem Sci       Date:  1997-06       Impact factor: 13.807

4.  PCR amplification of highly GC-rich DNA template after denaturation by NaOH.

Authors:  R K Agarwal; A Perl
Journal:  Nucleic Acids Res       Date:  1993-11-11       Impact factor: 16.971

5.  PCR-direct sequencing of a GC-rich region by inclusion of 10% DMSO: application to mouse c-jun.

Authors:  Y Sun; G Hegamyer; N H Colburn
Journal:  Biotechniques       Date:  1993-09       Impact factor: 1.993

6.  Quantitative RT-PCR for measuring gene expression.

Authors:  M C Riedy; E A Timm; C C Stewart
Journal:  Biotechniques       Date:  1995-01       Impact factor: 1.993

7.  Use of deoxyinosine in PCR to improve amplification of GC-rich DNA.

Authors:  S L Turner; F J Jenkins
Journal:  Biotechniques       Date:  1995-07       Impact factor: 1.993

8.  PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.

Authors:  W M Barnes
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-15       Impact factor: 11.205

9.  PCR bias in amplification of androgen receptor alleles, a trinucleotide repeat marker used in clonality studies.

Authors:  G L Mutter; K A Boynton
Journal:  Nucleic Acids Res       Date:  1995-04-25       Impact factor: 16.971

10.  Betaine can eliminate the base pair composition dependence of DNA melting.

Authors:  W A Rees; T D Yager; J Korte; P H von Hippel
Journal:  Biochemistry       Date:  1993-01-12       Impact factor: 3.162
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  122 in total

1.  In vivo, high-resolution analysis of yeast and mammalian RNA-protein interactions, RNA structure, RNA splicing and ribozyme cleavage by use of terminal transferase-dependent PCR.

Authors:  H H Chen; D Castanotto; J M LeBon; J J Rossi; A D Riggs
Journal:  Nucleic Acids Res       Date:  2000-04-01       Impact factor: 16.971

2.  Panfungal PCR and multiplex liquid hybridization for detection of fungi in tissue specimens.

Authors:  P H Hendolin; L Paulin; P Koukila-Kähkölä; V J Anttila; H Malmberg; M Richardson; J Ylikoski
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

3.  Two-step cycle sequencing improves base ambiguities and signal dropouts in DNA sequencing reactions using energy-transfer-based fluorescent dye terminators.

Authors:  L Wen
Journal:  Mol Biotechnol       Date:  2001-02       Impact factor: 2.695

4.  Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen.

Authors:  R R Duncan; D K Apps; M P Learmonth; M J Shipston; R H Chow
Journal:  Biochem J       Date:  2000-10-01       Impact factor: 3.857

5.  Three color cDNA microarrays: quantitative assessment through the use of fluorescein-labeled probes.

Authors:  Martin J Hessner; Xujing Wang; Katie Hulse; Lisa Meyer; Yan Wu; Steven Nye; Sun-Wei Guo; Soumitra Ghosh
Journal:  Nucleic Acids Res       Date:  2003-02-15       Impact factor: 16.971

6.  Use of a three-color cDNA microarray platform to measure and control support-bound probe for improved data quality and reproducibility.

Authors:  Martin J Hessner; Xujing Wang; Shehnaz Khan; Lisa Meyer; Michael Schlicht; Jennifer Tackes; Milton W Datta; Howard J Jacob; Soumitra Ghosh
Journal:  Nucleic Acids Res       Date:  2003-06-01       Impact factor: 16.971

Review 7.  Pre-PCR processing: strategies to generate PCR-compatible samples.

Authors:  Peter Rådström; Rickard Knutsson; Petra Wolffs; Maria Lövenklev; Charlotta Löfström
Journal:  Mol Biotechnol       Date:  2004-02       Impact factor: 2.695

8.  Bovine herpesvirus type 2 is closely related to the primate alphaherpesviruses.

Authors:  B Ehlers; M Goltz; M P Ejercito; G K Dasika; G J Letchworth
Journal:  Virus Genes       Date:  1999       Impact factor: 2.332

9.  Formation of pseudo-terminal restriction fragments, a PCR-related bias affecting terminal restriction fragment length polymorphism analysis of microbial community structure.

Authors:  Markus Egert; Michael W Friedrich
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

10.  Probing the transcription mechanisms of reovirus cores with molecules that alter RNA duplex stability.

Authors:  Alexander A Demidenko; Max L Nibert
Journal:  J Virol       Date:  2009-03-18       Impact factor: 5.103
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