Literature DB >> 9204710

Optimizing multiplex and LA-PCR with betaine.

P N Hengen1.   

Abstract

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and 'long and accurate' polymerase chain reaction (LA-PCR). For details on how to partake in the newsgroup, see the accompanying box.

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Year:  1997        PMID： 9204710     DOI： 10.1016/s0968-0004(97)01069-4

Source DB:  PubMed          Journal:  Trends Biochem Sci        ISSN： 0968-0004            Impact factor:   13.807


  12 in total

Review 1.  Multiplex PCR: optimization and application in diagnostic virology.

Authors:  E M Elnifro; A M Ashshi; R J Cooper; P E Klapper
Journal:  Clin Microbiol Rev       Date:  2000-10       Impact factor: 26.132

2.  New specificity and yield enhancer of polymerase chain reactions.

Authors:  M Kovárová; P Dráber
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

Review 3.  Reverse genetic approaches for functional genomics of rice.

Authors:  Gynheung An; Dong-Hoon Jeong; Ki-Hong Jung; Sichul Lee
Journal:  Plant Mol Biol       Date:  2005-09       Impact factor: 4.076

4.  Utility of amplification enhancers in low copy number DNA analysis.

Authors:  Pamela L Marshall; Jonathan L King; Bruce Budowle
Journal:  Int J Legal Med       Date:  2014-05-22       Impact factor: 2.686

5.  Enhanced protocol for determining the 3' terminus of hepatitis C virus.

Authors:  Xiaoan Zhang; Xiaofeng Fan; Yanjuan Xu; Adrian M Di Bisceglie
Journal:  J Virol Methods       Date:  2010-04-08       Impact factor: 2.014

6.  Betaine improves the PCR amplification of GC-rich DNA sequences.

Authors:  W Henke; K Herdel; K Jung; D Schnorr; S A Loening
Journal:  Nucleic Acids Res       Date:  1997-10-01       Impact factor: 16.971

7.  Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences.

Authors:  Marco Musso; Renata Bocciardi; Sara Parodi; Roberto Ravazzolo; Isabella Ceccherini
Journal:  J Mol Diagn       Date:  2006-11       Impact factor: 5.568

8.  Inhibitory effects of choline-O-sulfate on amyloid formation of human islet amyloid polypeptide.

Authors:  Mamoru Hagihara; Ayaka Takei; Takeshi Ishii; Fumio Hayashi; Kenji Kubota; Kaori Wakamatsu; Nobukazu Nameki
Journal:  FEBS Open Bio       Date:  2012-02-28       Impact factor: 2.693

9.  Compatible solute influence on nucleic acids: many questions but few answers.

Authors:  Matthias Kurz
Journal:  Saline Syst       Date:  2008-06-03

10.  Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I.

Authors:  Gouse M Shaik; Lubica Dráberová; Peter Dráber; Michael Boubelík; Petr Dráber
Journal:  Nucleic Acids Res       Date:  2008-07-07       Impact factor: 16.971
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