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Literature DB >> 2259646

Formamide can dramatically improve the specificity of PCR.

G Sarkar¹, S Kapelner, S S Sommer.

Abstract

Entities: Chemical

Mesh：

Substances：

Year: 1990 PMID： 2259646 PMCID： PMC332902 DOI： 10.1093/nar/18.24.7465

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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6 in total

1. A specificity enhancer for polymerase chain reaction.

Authors: T Hung; K Mak; K Fong
Journal: Nucleic Acids Res Date: 1990-08-25 Impact factor: 16.971

2. Cloning of the cDNA and gene for a human D2 dopamine receptor.

Authors: D K Grandy; M A Marchionni; H Makam; R E Stofko; M Alfano; L Frothingham; J B Fischer; K J Burke-Howie; J R Bunzow; A C Server
Journal: Proc Natl Acad Sci U S A Date: 1989-12 Impact factor: 11.205

3. Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity.

Authors: G Sarkar; S S Sommer
Journal: Science Date: 1989-04-21 Impact factor: 47.728

4. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

5. Structure-independent DNA amplification by PCR using 7-deaza-2'-deoxyguanosine.

Authors: L McConlogue; M A Brow; M A Innis
Journal: Nucleic Acids Res Date: 1988-10-25 Impact factor: 16.971

6. Specific amplification with PCR of a refractory segment of genomic DNA.

Authors: T M Haqqi; G Sarkar; C S David; S S Sommer
Journal: Nucleic Acids Res Date: 1988-12-23 Impact factor: 16.971

6 in total

62 in total

1. High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR.

Authors: B Fuchs; K Zhang; M G Rock; M E Bolander; G Sarkar
Journal: Mol Biotechnol Date: 1999-10 Impact factor: 2.695

2. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat.

Authors: W Abu Al-Soud; P Rådström
Journal: J Clin Microbiol Date: 2000-12 Impact factor: 5.948

3. New specificity and yield enhancer of polymerase chain reactions.

Authors: M Kovárová; P Dráber
Journal: Nucleic Acids Res Date: 2000-07-01 Impact factor: 16.971

4. Two-step cycle sequencing improves base ambiguities and signal dropouts in DNA sequencing reactions using energy-transfer-based fluorescent dye terminators.

Authors: L Wen
Journal: Mol Biotechnol Date: 2001-02 Impact factor: 2.695

5. Use of degenerate primers for polymerase chain reaction cloning and sequencing of the Lactococcus lactis subsp. lactis recA gene.

Authors: P Duwat; S D Ehrlich; A Gruss
Journal: Appl Environ Microbiol Date: 1992-08 Impact factor: 4.792