Literature DB >> 9380516

In vitro suppression as a tool for the investigation of translation initiation.

V A Karginov1, S V Mamaev, S M Hecht.   

Abstract

An in vitro protein synthesizing system that employs rabbit reticulocyte lysates has been employed for protein production from mRNAs containing nonsense (UAG) codons in the presence of misacylated suppressor tRNAs.The system includes a misacylated Escherichia coli tRNAAlaCUA that functions at least as efficiently as any suppressor tRNA transcript reported to date and which has been shown not to be a substrate for (re)activation by alanyl-tRNA synthetase. Application of the optimized system for preparation of dihydrofolate analogs has also permitted analysis of competing mechanisms that control the sites(s) of translation initiation.

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Year:  1997        PMID： 9380516      PMCID： PMC146976          DOI： 10.1093/nar/25.19.3912

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  39 in total

1.  Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.

Authors:  J R Sampson; O C Uhlenbeck
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

2.  At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells.

Authors:  M Kozak
Journal:  J Mol Biol       Date:  1987-08-20       Impact factor: 5.469

3.  Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes.

Authors:  M Kozak
Journal:  Mol Cell Biol       Date:  1987-10       Impact factor: 4.272

Review 4.  An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.

Authors:  M Kozak
Journal:  Nucleic Acids Res       Date:  1987-10-26       Impact factor: 16.971

5.  A simple structural feature is a major determinant of the identity of a transfer RNA.

Authors:  Y M Hou; P Schimmel
Journal:  Nature       Date:  1988-05-12       Impact factor: 49.962

6.  Mischarging Escherichia coli tRNAPhe with L-4'-[3-(trifluoromethyl)-3H-diazirin-3-yl]phenylalanine, a photoactivatable analogue of phenylalanine.

Authors:  G Baldini; B Martoglio; A Schachenmann; C Zugliani; J Brunner
Journal:  Biochemistry       Date:  1988-10-04       Impact factor: 3.162

7.  Ribosomal binding and dipeptide formation by misacylated tRNA(Phe),S.

Authors:  T G Heckler; J R Roesser; C Xu; P I Chang; S M Hecht
Journal:  Biochemistry       Date:  1988-09-20       Impact factor: 3.162

8.  T4 RNA ligase mediated preparation of novel "chemically misacylated" tRNAPheS.

Authors:  T G Heckler; L H Chang; Y Zama; T Naka; M S Chorghade; S M Hecht
Journal:  Biochemistry       Date:  1984-03-27       Impact factor: 3.162

9.  Escherichia coli tryptophan synthase: synthesis of catalytically competent alpha subunit in a cell-free system containing preacylated tRNAs.

Authors:  R C Payne; B P Nichols; S M Hecht
Journal:  Biochemistry       Date:  1987-06-02       Impact factor: 3.162

10.  Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.

Authors:  M Kozak
Journal:  Cell       Date:  1986-01-31       Impact factor: 41.582
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  2 in total

1.  Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery.

Authors:  Erwin Ilegems; Horst M Pick; Horst Vogel
Journal:  Nucleic Acids Res       Date:  2002-12-01       Impact factor: 16.971

2.  Five-base codons for incorporation of nonnatural amino acids into proteins.

Authors:  T Hohsaka; Y Ashizuka; H Murakami; M Sisido
Journal:  Nucleic Acids Res       Date:  2001-09-01       Impact factor: 16.971
  2 in total

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